Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   Q9R16_RS04400 Genome accession   NZ_CP133989
Coordinates   969240..970298 (-) Length   352 a.a.
NCBI ID   WP_046432299.1    Uniprot ID   A0A6N7NUT5
Organism   Stenotrophomonas sp. 9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 964240..975298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9R16_RS04380 (Q9R16_04380) - 965181..967151 (-) 1971 WP_142235229.1 type IV pilus secretin PilQ family protein -
  Q9R16_RS04385 (Q9R16_04385) - 967173..967706 (-) 534 WP_005418914.1 pilus assembly protein PilP -
  Q9R16_RS04390 (Q9R16_04390) pilO 967706..968362 (-) 657 WP_005418916.1 type 4a pilus biogenesis protein PilO -
  Q9R16_RS04395 (Q9R16_04395) - 968359..969240 (-) 882 WP_014648259.1 PilN domain-containing protein -
  Q9R16_RS04400 (Q9R16_04400) pilM 969240..970298 (-) 1059 WP_046432299.1 pilus assembly protein PilM Machinery gene
  Q9R16_RS04405 (Q9R16_04405) - 970518..972941 (+) 2424 WP_014648261.1 penicillin-binding protein 1A -
  Q9R16_RS04410 (Q9R16_04410) - 973154..973798 (+) 645 WP_014648262.1 hypothetical protein -
  Q9R16_RS04415 (Q9R16_04415) - 974398..975144 (-) 747 WP_014648263.1 membrane protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37727.33 Da        Isoelectric Point: 4.4006

>NTDB_id=878196 Q9R16_RS04400 WP_046432299.1 969240..970298(-) (pilM) [Stenotrophomonas sp. 9]
MGLIPKSQSPLVGVDISSTAVKLLQLSRSGNRFRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIRRAMNRSGSKAKVAAAA
VAGSAVITKVIPMPADLDENDMEAQIELEAVNYIPYPIEEVNLDFEVIGSIPNNPEMVQVLLAASRSENVELRQSALELG
GLQAKVMDVEAFAVENAYALVASELPVSIDGVVALVDIGATMTTLNVLRGGRSLYSREQVFGGKQLTDEIMRRYGLSYEE
AGLAKRQGGLPESYEMEVLEPFKEATVQQISRLLQFFYAGSEFNRVDHIVLAGGCAVLGGLPEMVEEQLGVPTVVANPLA
QMTLGPKVNAHALAQDAPALMIATGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=878196 Q9R16_RS04400 WP_046432299.1 969240..970298(-) (pilM) [Stenotrophomonas sp. 9]
GTGGGGCTCATCCCAAAAAGTCAGTCGCCGCTCGTAGGCGTCGACATCAGTTCGACTGCGGTAAAGCTTTTGCAGCTGTC
CCGCAGCGGCAATCGTTTCCGCGTGGAACATTACGCTGTGGAACCTCTGCCGCCCAATGCGGTGGTGGAGAAGAACATCG
TTGAAGTGGAGGCCGTGGGTGAGGCCATCCGTCGGGCGATGAACCGTTCAGGCAGCAAGGCCAAAGTGGCCGCTGCCGCC
GTCGCGGGGTCGGCAGTGATCACCAAGGTGATCCCGATGCCGGCCGATCTCGACGAAAACGACATGGAAGCCCAGATCGA
GCTGGAAGCGGTCAACTACATTCCGTATCCGATCGAGGAAGTGAACCTGGACTTCGAGGTGATCGGGTCGATCCCGAACA
ACCCGGAGATGGTCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAATGTCGAGCTGCGCCAGTCCGCGCTGGAACTCGGT
GGCCTGCAGGCCAAGGTGATGGACGTGGAGGCCTTCGCGGTCGAGAACGCCTACGCCCTGGTCGCCAGCGAGCTGCCGGT
ATCCATCGACGGCGTGGTCGCGCTGGTCGATATCGGCGCCACCATGACCACCCTCAATGTCCTGCGCGGCGGCCGCAGCC
TCTACAGCCGCGAGCAGGTGTTCGGTGGCAAGCAGCTGACCGACGAGATCATGCGCCGCTACGGGCTGAGCTACGAGGAA
GCCGGGCTGGCCAAGCGCCAGGGCGGCCTGCCGGAAAGCTACGAGATGGAAGTGCTGGAGCCGTTCAAGGAAGCCACGGT
CCAGCAGATCAGCCGCCTGCTGCAGTTCTTCTACGCAGGCAGCGAATTCAACCGCGTCGATCACATCGTGCTGGCCGGCG
GCTGCGCCGTGCTCGGTGGCCTGCCGGAGATGGTCGAGGAACAGCTGGGCGTGCCGACCGTGGTCGCCAACCCGCTGGCG
CAGATGACCCTGGGGCCGAAGGTGAACGCGCATGCGTTGGCCCAGGATGCCCCCGCGCTGATGATCGCGACCGGTCTGGC
GCTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N7NUT5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.42

100

0.514
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.284

100

0.503
  pilM Legionella pneumophila strain ERS1305867

47.863

99.716

0.477