Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RHS38_RS01590 Genome accession   NZ_CP133891
Coordinates   336707..337300 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain M0904     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 331707..342300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS38_RS01575 (RHS38_01575) - 332423..334993 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  RHS38_RS01580 (RHS38_01580) pilM 335136..336152 (+) 1017 WP_085609516.1 type IV pilus biogenesis protein PilM Machinery gene
  RHS38_RS01585 (RHS38_01585) pilN 336136..336714 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  RHS38_RS01590 (RHS38_01590) pilO 336707..337300 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  RHS38_RS01595 (RHS38_01595) pilP 337290..337805 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  RHS38_RS01600 (RHS38_01600) pilQ 337837..339585 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  RHS38_RS01605 (RHS38_01605) aroK 339774..340292 (+) 519 WP_005381319.1 shikimate kinase AroK -
  RHS38_RS01610 (RHS38_01610) aroB 340324..341424 (+) 1101 WP_025546399.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=877371 RHS38_RS01590 WP_005480482.1 336707..337300(+) (pilO) [Vibrio parahaemolyticus strain M0904]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=877371 RHS38_RS01590 WP_005480482.1 336707..337300(+) (pilO) [Vibrio parahaemolyticus strain M0904]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTAGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CGCTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726