Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RHS38_RS01580 Genome accession   NZ_CP133891
Coordinates   335136..336152 (+) Length   338 a.a.
NCBI ID   WP_085609516.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain M0904     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 330136..341152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHS38_RS01565 (RHS38_01565) - 330560..331288 (-) 729 WP_057619621.1 redoxin family protein -
  RHS38_RS01570 (RHS38_01570) oxyR 331459..332367 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  RHS38_RS01575 (RHS38_01575) - 332423..334993 (-) 2571 WP_005496567.1 penicillin-binding protein 1A -
  RHS38_RS01580 (RHS38_01580) pilM 335136..336152 (+) 1017 WP_085609516.1 type IV pilus biogenesis protein PilM Machinery gene
  RHS38_RS01585 (RHS38_01585) pilN 336136..336714 (+) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  RHS38_RS01590 (RHS38_01590) pilO 336707..337300 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  RHS38_RS01595 (RHS38_01595) pilP 337290..337805 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  RHS38_RS01600 (RHS38_01600) pilQ 337837..339585 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ Machinery gene
  RHS38_RS01605 (RHS38_01605) aroK 339774..340292 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37725.23 Da        Isoelectric Point: 6.6634

>NTDB_id=877369 RHS38_RS01580 WP_085609516.1 335136..336152(+) (pilM) [Vibrio parahaemolyticus strain M0904]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
SQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAMRGIHWLGGVRAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=877369 RHS38_RS01580 WP_085609516.1 335136..336152(+) (pilM) [Vibrio parahaemolyticus strain M0904]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCCAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAAGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATTTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATCTCAATGGGGACGTCACAAGAAGACTTACTTTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
AGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGCCGAGCGGCCGATTGGCAACATTTTTCGACGGCTGCA
GGGCTGGCGATGCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482