Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RDJ20_RS26150 Genome accession   NZ_CP133755
Coordinates   5457323..5458543 (+) Length   406 a.a.
NCBI ID   WP_033976387.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain BJ86     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5452323..5463543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RDJ20_RS26130 (RDJ20_26130) nadC 5453895..5454743 (+) 849 WP_033976390.1 carboxylating nicotinate-nucleotide diphosphorylase -
  RDJ20_RS26140 (RDJ20_26140) pilA 5454932..5455387 (-) 456 WP_033976389.1 pilin Machinery gene
  RDJ20_RS26145 (RDJ20_26145) pilB 5455619..5457319 (+) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RDJ20_RS26150 (RDJ20_26150) pilC 5457323..5458543 (+) 1221 WP_033976387.1 type 4a pilus biogenesis protein PilC Machinery gene
  RDJ20_RS26155 (RDJ20_26155) pilD 5458547..5459419 (+) 873 WP_033976386.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  RDJ20_RS26160 (RDJ20_26160) coaE 5459416..5460027 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  RDJ20_RS26165 (RDJ20_26165) yacG 5460024..5460224 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  RDJ20_RS26170 (RDJ20_26170) - 5460261..5460470 (-) 210 WP_003094660.1 hypothetical protein -
  RDJ20_RS26175 (RDJ20_26175) - 5460576..5461265 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  RDJ20_RS26180 (RDJ20_26180) - 5461262..5461732 (-) 471 WP_033970459.1 hypothetical protein -
  RDJ20_RS26185 (RDJ20_26185) - 5461729..5462154 (-) 426 WP_033976385.1 GNAT family N-acetyltransferase -
  RDJ20_RS26190 (RDJ20_26190) - 5462287..5462916 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  RDJ20_RS26195 (RDJ20_26195) - 5462913..5463361 (+) 449 Protein_5185 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44330.12 Da        Isoelectric Point: 9.8784

>NTDB_id=876346 RDJ20_RS26150 WP_033976387.1 5457323..5458543(+) (pilC) [Pseudomonas aeruginosa strain BJ86]
MAEKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSKKFRDALDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=876346 RDJ20_RS26150 WP_033976387.1 5457323..5458543(+) (pilC) [Pseudomonas aeruginosa strain BJ86]
ATGGCGGAAAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAAGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACATACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTGCTAATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACGCACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTAGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCTTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCTATCGGCGAGGAGTCCGG
TTCGCTCGACGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAGCCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baylyi ADP1

60.345

100

0.603
  pilC Acinetobacter baumannii D1279779

60.494

99.754

0.603
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.604

98.276

0.409
  pilC Thermus thermophilus HB27

36.908

98.768

0.365