Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   RFN66_RS00595 Genome accession   NZ_CP133705
Coordinates   108514..109893 (+) Length   459 a.a.
NCBI ID   WP_020449839.1    Uniprot ID   A0AAW6KF37
Organism   Bacillus paralicheniformis strain CP47     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 103514..114893
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN66_RS00575 (RFN66_00575) - 103881..104345 (+) 465 WP_020449835.1 CtsR family transcriptional regulator -
  RFN66_RS00580 (RFN66_00580) - 104360..104914 (+) 555 WP_003178271.1 UvrB/UvrC motif-containing protein -
  RFN66_RS00585 (RFN66_00585) - 104914..106005 (+) 1092 WP_023855926.1 protein arginine kinase -
  RFN66_RS00590 (RFN66_00590) clpC 106002..108434 (+) 2433 WP_020449838.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  RFN66_RS00595 (RFN66_00595) radA 108514..109893 (+) 1380 WP_020449839.1 DNA repair protein RadA Machinery gene
  RFN66_RS00600 (RFN66_00600) disA 109897..110973 (+) 1077 WP_023855924.1 DNA integrity scanning diadenylate cyclase DisA -
  RFN66_RS00605 (RFN66_00605) - 111105..112193 (+) 1089 WP_009330350.1 PIN/TRAM domain-containing protein -
  RFN66_RS00610 (RFN66_00610) ispD 112210..112905 (+) 696 WP_020449841.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  RFN66_RS00615 (RFN66_00615) ispF 112898..113374 (+) 477 WP_020449842.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49848.28 Da        Isoelectric Point: 7.4339

>NTDB_id=875879 RFN66_RS00595 WP_020449839.1 108514..109893(+) (radA) [Bacillus paralicheniformis strain CP47]
MAKTKTKFICHSCGYESAKWMGKCPGCGTWNTMVEETIKKPAANRRTAFSHSVQTVQKPSPLTSIETTEEPRIKTKLGEF
NRVLGNGIVKGSLVLIGGDPGIGKSTLLLQVSAQLADSKENVLYISGEESVKQTKLRADRLGINSPTLHVLSETDMEYIT
SSIEEMNPSFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMRIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGAAGSSVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDTPPHPTDCFIGEVGLT
GEVRRVSRIEQRVQEAAKLGFKRMIIPSANVEGWTKPKGIEVVGVENVAEALRASLGGS

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=875879 RFN66_RS00595 WP_020449839.1 108514..109893(+) (radA) [Bacillus paralicheniformis strain CP47]
ATGGCTAAAACAAAGACTAAATTCATTTGTCACTCATGCGGTTACGAATCCGCCAAGTGGATGGGGAAGTGCCCCGGATG
CGGCACATGGAATACCATGGTGGAAGAAACGATAAAAAAACCCGCCGCCAACAGAAGAACCGCTTTTTCACATTCCGTTC
AAACGGTGCAAAAGCCTTCACCTCTCACTTCAATCGAAACAACAGAAGAGCCGCGAATTAAAACGAAACTGGGCGAATTC
AACCGCGTCTTGGGAAACGGTATTGTCAAAGGTTCACTCGTTTTAATCGGAGGCGATCCCGGCATCGGGAAATCAACCTT
GCTGCTTCAAGTATCTGCCCAGCTCGCTGATTCAAAAGAAAATGTCCTGTACATCTCAGGTGAAGAATCGGTCAAGCAGA
CAAAGCTGAGAGCAGACCGTCTAGGCATCAACAGTCCCACTCTTCACGTTTTATCTGAAACCGATATGGAGTATATTACG
TCTTCTATAGAAGAGATGAATCCATCATTCGTGGTGGTTGATTCGATTCAAACCGTTTACCAAAGTGATATTACATCTGC
TCCAGGCAGCGTGTCCCAGGTCAGGGAATGCACCGCTGAGCTGATGAGAATTGCAAAAACAAAAGGGATTCCGATATTTA
TCGTCGGGCATGTCACAAAAGAAGGTTCGATTGCCGGACCGAGACTTCTGGAACATATGGTCGACACCGTCCTTTATTTT
GAAGGTGAGCGGCATCATACATTTCGGATTTTAAGAGCCGTCAAAAACCGGTTTGGATCAACGAATGAAATGGGAATCTT
TGAAATGAGGGAAGAGGGCCTAACAGAAGTGCTGAATCCGTCGGAGATCTTTCTCGAAGAGCGCTCGGCTGGAGCAGCCG
GCTCGAGTGTCGTGGCTTCAATGGAAGGCACGAGGCCGGTCTTAGTAGAGATTCAGGCGCTGATTTCCCCGACGAGTTTT
GGAAATCCGCGCAGGATGGCAACCGGAATTGATCATAACCGCGTCTCATTGCTGATGGCGGTTTTAGAGAAAAGGGTTGG
GCTGCTGCTGCAAAATCAAGACGCCTATTTAAAAGTCGCCGGCGGCGTCAAGCTGGACGAGCCGGCGATCGACCTCGCCG
TTGCTGTGAGCATCGCCTCAAGCTTCAGAGACACCCCGCCTCATCCGACGGATTGTTTTATCGGCGAAGTCGGCTTGACA
GGGGAAGTCCGCAGAGTATCAAGGATAGAACAGAGGGTGCAGGAAGCGGCGAAGCTTGGTTTTAAAAGAATGATTATTCC
TTCTGCAAATGTGGAAGGATGGACAAAGCCGAAAGGAATTGAAGTCGTCGGCGTTGAAAATGTAGCTGAGGCCCTTCGAG
CTTCATTAGGAGGATCATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(66-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

91.048

99.782

0.908
  radA Streptococcus mitis NCTC 12261

62.031

98.693

0.612
  radA Streptococcus pneumoniae Rx1

62.031

98.693

0.612
  radA Streptococcus pneumoniae D39

62.031

98.693

0.612
  radA Streptococcus pneumoniae R6

62.031

98.693

0.612
  radA Streptococcus pneumoniae TIGR4

62.031

98.693

0.612
  radA Streptococcus mitis SK321

61.81

98.693

0.61