Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   RFN65_RS08500 Genome accession   NZ_CP133703
Coordinates   1652428..1652775 (+) Length   115 a.a.
NCBI ID   WP_063335293.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1647428..1657775
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS08475 (RFN65_08475) corA 1648256..1649209 (+) 954 WP_309530235.1 magnesium transporter CorA -
  RFN65_RS08480 (RFN65_08480) comGA 1649619..1650689 (+) 1071 WP_015483431.1 competence protein ComGA Machinery gene
  RFN65_RS08485 (RFN65_08485) comGB 1650676..1651713 (+) 1038 WP_047182873.1 comG operon protein ComGB Machinery gene
  RFN65_RS08490 (RFN65_08490) comGC 1651727..1652023 (+) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  RFN65_RS08495 (RFN65_08495) comGD 1652013..1652444 (+) 432 WP_015384089.1 comG operon protein ComGD Machinery gene
  RFN65_RS08500 (RFN65_08500) comGE 1652428..1652775 (+) 348 WP_063335293.1 ComG operon protein 5 Machinery gene
  RFN65_RS08505 (RFN65_08505) comGF 1652801..1653184 (+) 384 WP_063335292.1 ComG operon protein ComGF Machinery gene
  RFN65_RS08510 (RFN65_08510) comGG 1653185..1653559 (+) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  RFN65_RS08515 (RFN65_08515) spoIITA 1653631..1653810 (+) 180 WP_003230176.1 YqzE family protein -
  RFN65_RS08520 (RFN65_08520) yqzG 1653852..1654178 (-) 327 WP_047183570.1 YqzG/YhdC family protein -
  RFN65_RS08525 (RFN65_08525) tapA 1654448..1655209 (+) 762 WP_063335290.1 amyloid fiber anchoring/assembly protein TapA -
  RFN65_RS08530 (RFN65_08530) sipW 1655193..1655765 (+) 573 WP_080030740.1 signal peptidase I SipW -
  RFN65_RS08535 (RFN65_08535) tasA 1655829..1656614 (+) 786 WP_003230183.1 biofilm matrix protein TasA -
  RFN65_RS08540 (RFN65_08540) sinR 1656707..1657042 (-) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  RFN65_RS08545 (RFN65_08545) sinI 1657076..1657249 (-) 174 WP_003230187.1 anti-repressor SinI Regulator

Sequence

Protein


Download         Length: 115 a.a.        Molecular weight: 13287.32 Da        Isoelectric Point: 5.1901

>NTDB_id=875830 RFN65_RS08500 WP_063335293.1 1652428..1652775(+) (comGE) [Bacillus subtilis strain CP35]
MWRENKGFSTIETMSALSLWLFVLLTVVPLWDKLMSDEKMAESREIGYQMMNESISKYVMSGEGAASKTITKNNHIYAMK
WEEEGEYQNVCIKAAAYKEKSFCLSILQTEWLHAS

Nucleotide


Download         Length: 348 bp        

>NTDB_id=875830 RFN65_RS08500 WP_063335293.1 1652428..1652775(+) (comGE) [Bacillus subtilis strain CP35]
ATGTGGAGAGAAAATAAAGGTTTTTCTACAATAGAAACAATGTCAGCGCTAAGCCTGTGGCTGTTTGTGCTGCTGACAGT
CGTCCCCTTGTGGGACAAGCTGATGTCTGATGAAAAAATGGCGGAATCACGAGAAATTGGCTATCAGATGATGAATGAGA
GCATTAGCAAATATGTCATGAGTGGTGAAGGAGCCGCGTCAAAAACGATTACAAAGAACAATCATATCTATGCAATGAAG
TGGGAGGAGGAGGGCGAATATCAAAACGTATGTATCAAAGCCGCAGCTTATAAAGAAAAATCATTTTGCCTCAGCATTTT
GCAGACAGAATGGCTACACGCTTCTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Bacillus subtilis subsp. subtilis str. 168

99.13

100

0.991