Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RFN65_RS08040 Genome accession   NZ_CP133703
Coordinates   1569591..1570208 (+) Length   205 a.a.
NCBI ID   WP_063335454.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1564591..1575208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS08005 (RFN65_08005) aroE 1565249..1566091 (+) 843 WP_309530224.1 shikimate dehydrogenase -
  RFN65_RS08010 (RFN65_08010) yhbY 1566085..1566375 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  RFN65_RS08015 (RFN65_08015) nadD 1566387..1566956 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  RFN65_RS08020 (RFN65_08020) yqeK 1566946..1567506 (+) 561 WP_015483448.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RFN65_RS08025 (RFN65_08025) rsfS 1567524..1567880 (+) 357 WP_041333759.1 ribosome silencing factor -
  RFN65_RS08030 (RFN65_08030) yqeM 1567877..1568620 (+) 744 WP_015384134.1 class I SAM-dependent methyltransferase -
  RFN65_RS08035 (RFN65_08035) comER 1568686..1569507 (-) 822 WP_063335455.1 late competence protein ComER -
  RFN65_RS08040 (RFN65_08040) comEA 1569591..1570208 (+) 618 WP_063335454.1 competence protein ComEA Machinery gene
  RFN65_RS08045 (RFN65_08045) comEB 1570275..1570844 (+) 570 WP_003229978.1 ComE operon protein 2 -
  RFN65_RS08050 (RFN65_08050) comEC 1570848..1573178 (+) 2331 WP_309530225.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RFN65_RS08055 (RFN65_08055) yqzM 1573218..1573352 (-) 135 WP_003229983.1 YqzM family protein -
  RFN65_RS08060 (RFN65_08060) - 1573393..1573542 (+) 150 WP_003229985.1 hypothetical protein -
  RFN65_RS08065 (RFN65_08065) holA 1573582..1574625 (+) 1044 WP_014664666.1 DNA polymerase III subunit delta -
  RFN65_RS08070 (RFN65_08070) rpsT 1574640..1574906 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21762.55 Da        Isoelectric Point: 4.9552

>NTDB_id=875822 RFN65_RS08040 WP_063335454.1 1569591..1570208(+) (comEA) [Bacillus subtilis strain CP35]
MNWLNQHKKAIILAASAAVFTAIMIVLATGKNKEPVKQAVPTETENTVVKQEANNDKSNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=875822 RFN65_RS08040 WP_063335454.1 1569591..1570208(+) (comEA) [Bacillus subtilis strain CP35]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCGTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACAAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAGAACGGTCGTTTCCAAACAATTGAAGATATTACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

35.909

100

0.385