Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RFN65_RS07960 Genome accession   NZ_CP133703
Coordinates   1557924..1558334 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain CP35     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1552924..1563334
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS07930 (RFN65_07930) yrkP 1554028..1554723 (+) 696 WP_309530220.1 two-component response regulator YrkP -
  RFN65_RS07935 (RFN65_07935) yrkQ 1554710..1556008 (+) 1299 WP_015483453.1 two-component system sensor histidine kinase YrkQ -
  RFN65_RS07940 (RFN65_07940) - 1556053..1556148 (+) 96 Protein_1559 phosphate-starvation-inducible protein PsiE -
  RFN65_RS07945 (RFN65_07945) - 1556605..1556769 (+) 165 WP_080467334.1 hypothetical protein -
  RFN65_RS07950 (RFN65_07950) - 1556727..1556918 (+) 192 Protein_1561 recombinase family protein -
  RFN65_RS07955 (RFN65_07955) sigK 1557000..1557728 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  RFN65_RS07960 (RFN65_07960) nucA/comI 1557924..1558334 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  RFN65_RS07965 (RFN65_07965) yqeB 1558367..1559089 (-) 723 WP_309530221.1 DUF308 domain-containing protein -
  RFN65_RS07970 (RFN65_07970) gnd 1559341..1560234 (+) 894 WP_047182906.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  RFN65_RS07975 (RFN65_07975) yqeD 1560253..1560879 (-) 627 WP_309530222.1 TVP38/TMEM64 family protein -
  RFN65_RS07980 (RFN65_07980) cwlH 1561066..1561818 (+) 753 WP_309530223.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  RFN65_RS07985 (RFN65_07985) yqeF 1562070..1562801 (+) 732 WP_047182903.1 SGNH/GDSL hydrolase family protein -
  RFN65_RS07990 (RFN65_07990) - 1563107..1563247 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=875821 RFN65_RS07960 WP_009967785.1 1557924..1558334(+) (nucA/comI) [Bacillus subtilis strain CP35]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=875821 RFN65_RS07960 WP_009967785.1 1557924..1558334(+) (nucA/comI) [Bacillus subtilis strain CP35]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGTGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529