Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   RFN65_RS02995 Genome accession   NZ_CP133703
Coordinates   592487..593878 (+) Length   463 a.a.
NCBI ID   WP_309530046.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 587487..598878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS02975 (RFN65_02975) yvyE 588639..589292 (-) 654 WP_003227979.1 YigZ family protein -
  RFN65_RS02980 (RFN65_02980) degS 589509..590666 (+) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  RFN65_RS02985 (RFN65_02985) degU 590749..591438 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  RFN65_RS02990 (RFN65_02990) fakBA 591536..592381 (+) 846 WP_309530045.1 DegV family protein -
  RFN65_RS02995 (RFN65_02995) comFA 592487..593878 (+) 1392 WP_309530046.1 ATP-dependent helicase ComFA Machinery gene
  RFN65_RS03000 (RFN65_03000) comFB 593938..594234 (+) 297 WP_015483764.1 late competence protein ComFB -
  RFN65_RS03005 (RFN65_03005) comFC 594198..594920 (+) 723 WP_157799620.1 comF operon protein ComFC Machinery gene
  RFN65_RS03010 (RFN65_03010) yvyF 594994..595413 (+) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  RFN65_RS03015 (RFN65_03015) flgM 595494..595760 (+) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  RFN65_RS03020 (RFN65_03020) flgN 595776..596258 (+) 483 WP_014481048.1 flagellar protein FlgN -
  RFN65_RS03025 (RFN65_03025) flgK 596277..597800 (+) 1524 WP_063334746.1 flagellar hook-associated protein FlgK -
  RFN65_RS03030 (RFN65_03030) flgL 597811..598707 (+) 897 WP_014478126.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52585.66 Da        Isoelectric Point: 9.9286

>NTDB_id=875806 RFN65_RS02995 WP_309530046.1 592487..593878(+) (comFA) [Bacillus subtilis strain CP35]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSNDKWRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SGDQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=875806 RFN65_RS02995 WP_309530046.1 592487..593878(+) (comFA) [Bacillus subtilis strain CP35]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGGAGCCGTCATTTGCTCAGGAC
TGAACTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGAAGTCAATTAAACTGACATGGGATGGCAAGCTTTCAAGCGGACAACAAAAAGCCGCCAATGTATTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCAATGACAAATGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGGTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCTGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.272

100

0.983