Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   RFN65_RS01905 Genome accession   NZ_CP133703
Coordinates   371969..372088 (-) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain CP35     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 366969..377088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS01885 (RFN65_01885) - 367640..368203 (-) 564 WP_015483840.1 hypothetical protein -
  RFN65_RS01890 (RFN65_01890) - 368196..368843 (-) 648 WP_015384940.1 ABC transporter ATP-binding protein -
  RFN65_RS01895 (RFN65_01895) - 370319..371005 (-) 687 WP_236822444.1 hypothetical protein -
  RFN65_RS01900 (RFN65_01900) - 371265..371741 (-) 477 WP_015384936.1 hypothetical protein -
  RFN65_RS01905 (RFN65_01905) phrF 371969..372088 (-) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  RFN65_RS01910 (RFN65_01910) rapF 372072..373217 (-) 1146 WP_015384935.1 response regulator aspartate phosphatase RapF Regulator
  RFN65_RS01915 (RFN65_01915) - 373220..373450 (+) 231 WP_015483838.1 hypothetical protein -
  RFN65_RS01920 (RFN65_01920) ywhK 373447..374802 (+) 1356 WP_309530021.1 YncE family protein -
  RFN65_RS01925 (RFN65_01925) ywhL 374842..376218 (+) 1377 WP_309530022.1 YncE family protein -
  RFN65_RS01930 (RFN65_01930) albG 376224..376925 (-) 702 WP_100505938.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=875799 RFN65_RS01905 WP_009968329.1 371969..372088(-) (phrF) [Bacillus subtilis strain CP35]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=875799 RFN65_RS01905 WP_009968329.1 371969..372088(-) (phrF) [Bacillus subtilis strain CP35]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487