Detailed information NTG0875800

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: RFN65_RS01910
• Genome accession: NZ_CP133703
• Coordinates: 372072..373217 (-)
• Length: 381 a.a.
• NCBI ID: WP_015384935.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain CP35
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 367072..378217

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RFN65_RS01885 (RFN65_01885)	-	367640..368203 (-)	564	WP_015483840.1	hypothetical protein	-
RFN65_RS01890 (RFN65_01890)	-	368196..368843 (-)	648	WP_015384940.1	ABC transporter ATP-binding protein	-
RFN65_RS01895 (RFN65_01895)	-	370319..371005 (-)	687	WP_236822444.1	hypothetical protein	-
RFN65_RS01900 (RFN65_01900)	-	371265..371741 (-)	477	WP_015384936.1	hypothetical protein	-
RFN65_RS01905 (RFN65_01905)	phrF	371969..372088 (-)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
RFN65_RS01910 (RFN65_01910)	rapF	372072..373217 (-)	1146	WP_015384935.1	response regulator aspartate phosphatase RapF	Regulator
RFN65_RS01915 (RFN65_01915)	-	373220..373450 (+)	231	WP_015483838.1	hypothetical protein	-
RFN65_RS01920 (RFN65_01920)	ywhK	373447..374802 (+)	1356	WP_309530021.1	YncE family protein	-
RFN65_RS01925 (RFN65_01925)	ywhL	374842..376218 (+)	1377	WP_309530022.1	YncE family protein	-
RFN65_RS01930 (RFN65_01930)	albG	376224..376925 (-)	702	WP_100505938.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
RFN65_RS01935 (RFN65_01935)	albF	376922..378202 (-)	1281	WP_309530023.1	pitrilysin family protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45617.08 Da
• Isoelectric Point: 5.1503

>NTDB_id=875800 RFN65_RS01910 WP_015384935.1 372072..373217(-) (rapF) [Bacillus subtilis strain CP35]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDHMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=875800 RFN65_RS01910 WP_015384935.1 372072..373217(-) (rapF) [Bacillus subtilis strain CP35]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTTAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCACATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTAATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAATTTCTTCAG
AGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAGAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.95	100	0.99
rapC	Bacillus subtilis subsp. subtilis str. 168	57.632	99.738	0.575