Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   RFF10_RS06350 Genome accession   NZ_CP133636
Coordinates   1386654..1387199 (-) Length   181 a.a.
NCBI ID   WP_046333479.1    Uniprot ID   -
Organism   Pasteurella multocida strain Past29     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1381654..1392199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFF10_RS06325 (RFF10_06325) - 1381865..1382302 (-) 438 WP_010907449.1 methyltransferase family protein -
  RFF10_RS06330 (RFF10_06330) - 1382514..1383125 (-) 612 WP_046333474.1 YfgM family protein -
  RFF10_RS06335 (RFF10_06335) hisS 1383144..1384415 (-) 1272 WP_046333477.1 histidine--tRNA ligase -
  RFF10_RS06340 (RFF10_06340) ispG 1384445..1385548 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  RFF10_RS06345 (RFF10_06345) - 1385575..1386531 (-) 957 WP_046333478.1 helix-turn-helix domain-containing protein -
  RFF10_RS06350 (RFF10_06350) pilF/pilF2 1386654..1387199 (-) 546 WP_046333479.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  RFF10_RS06355 (RFF10_06355) - 1387238..1388422 (-) 1185 WP_046333480.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  RFF10_RS06360 (RFF10_06360) - 1388603..1389856 (-) 1254 WP_005755688.1 AI-2E family transporter -
  RFF10_RS06365 (RFF10_06365) glnB 1389857..1390195 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  RFF10_RS06370 (RFF10_06370) mog 1390240..1390818 (-) 579 WP_046333482.1 molybdopterin adenylyltransferase -
  RFF10_RS06375 (RFF10_06375) - 1390844..1391773 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20829.28 Da        Isoelectric Point: 7.8917

>NTDB_id=875373 RFF10_RS06350 WP_046333479.1 1386654..1387199(-) (pilF/pilF2) [Pasteurella multocida strain Past29]
MNRTFRFGIASIFSLFLSACVSQEGNNTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=875373 RFF10_RS06350 WP_046333479.1 1386654..1387199(-) (pilF/pilF2) [Pasteurella multocida strain Past29]
ATGAACCGCACTTTTCGATTTGGGATCGCGAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
TACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

52.096

92.265

0.481
  pilF Glaesserella parasuis strain SC1401

44.865

100

0.459