Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   RE735_RS10990 Genome accession   NZ_CP133585
Coordinates   2024176..2024556 (-) Length   126 a.a.
NCBI ID   WP_234891323.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2019176..2029556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS10955 (RE735_10955) - 2020162..2020392 (-) 231 WP_008343816.1 DUF896 domain-containing protein -
  RE735_RS10960 (RE735_10960) - 2020462..2021109 (-) 648 WP_309173060.1 recombinase family protein -
  RE735_RS10965 (RE735_10965) yneA 2021127..2021438 (-) 312 WP_309173061.1 cell division suppressor protein YneA -
  RE735_RS10970 (RE735_10970) lexA 2021597..2022217 (+) 621 WP_007499350.1 transcriptional repressor LexA -
  RE735_RS10975 (RE735_10975) - 2022265..2022708 (-) 444 WP_309173062.1 hypothetical protein -
  RE735_RS10980 (RE735_10980) - 2023006..2023548 (+) 543 WP_309173063.1 IseA DL-endopeptidase inhibitor family protein -
  RE735_RS10985 (RE735_10985) - 2023581..2024113 (-) 533 Protein_2149 STAS domain-containing protein -
  RE735_RS10990 (RE735_10990) nucA/comI 2024176..2024556 (-) 381 WP_234891323.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  RE735_RS10995 (RE735_10995) - 2024784..2026778 (-) 1995 WP_309173064.1 glycosyltransferase family 39 protein -
  RE735_RS11000 (RE735_11000) - 2026925..2027833 (-) 909 WP_309173065.1 trypsin-like serine protease -
  RE735_RS11005 (RE735_11005) - 2028118..2029203 (-) 1086 WP_309173066.1 T4 RnlA family RNA ligase -

Sequence

Protein


Download         Length: 126 a.a.        Molecular weight: 13786.44 Da        Isoelectric Point: 7.9019

>NTDB_id=875114 RE735_RS10990 WP_234891323.1 2024176..2024556(-) (nucA/comI) [Bacillus aerius strain NEB1378]
MGVLLGGFGEDRSAKGADRYDHVIQFPKERYPETGSHIQEAIRKGHSDVCTIDRNGADARRQESLKGIPTKPGFDRDEWP
MAVCLEGGKGASVQYVSPSDNRGAGSWVGHQISGYPDGKRILFIVK

Nucleotide


Download         Length: 381 bp        

>NTDB_id=875114 RE735_RS10990 WP_234891323.1 2024176..2024556(-) (nucA/comI) [Bacillus aerius strain NEB1378]
ATGGGTGTGTTATTAGGGGGTTTTGGTGAGGATCGTTCCGCAAAAGGGGCTGATCGTTATGATCATGTGATTCAATTTCC
TAAGGAACGGTATCCTGAAACAGGCAGTCATATTCAAGAAGCTATTCGAAAAGGGCACTCAGACGTGTGTACCATTGACC
GAAATGGGGCAGATGCCCGCAGGCAAGAATCATTAAAAGGAATTCCCACAAAACCTGGTTTTGACCGGGATGAATGGCCG
ATGGCAGTGTGTCTTGAAGGAGGAAAGGGGGCGAGCGTTCAATATGTCAGTCCATCAGATAATAGAGGCGCTGGTTCATG
GGTCGGTCATCAAATCAGCGGGTATCCTGACGGGAAACGAATTTTATTTATTGTCAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(29-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.824

94.444

0.556