Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   RE735_RS03145 Genome accession   NZ_CP133585
Coordinates   617484..618863 (+) Length   459 a.a.
NCBI ID   WP_309174543.1    Uniprot ID   -
Organism   Bacillus aerius strain NEB1378     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 612484..623863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RE735_RS03125 (RE735_03125) - 613491..614159 (-) 669 WP_309174540.1 YigZ family protein -
  RE735_RS03130 (RE735_03130) degS 614381..615556 (+) 1176 WP_309174541.1 sensor histidine kinase Regulator
  RE735_RS03135 (RE735_03135) degU 615620..616309 (+) 690 WP_008348273.1 two-component system response regulator DegU Regulator
  RE735_RS03140 (RE735_03140) - 616522..617364 (+) 843 WP_309174542.1 DegV family protein -
  RE735_RS03145 (RE735_03145) comFA 617484..618863 (+) 1380 WP_309174543.1 DEAD/DEAH box helicase Machinery gene
  RE735_RS03150 (RE735_03150) - 618912..619178 (+) 267 WP_309174544.1 late competence development ComFB family protein -
  RE735_RS03155 (RE735_03155) - 619139..619861 (+) 723 WP_309174545.1 ComF family protein -
  RE735_RS03160 (RE735_03160) - 619918..620337 (+) 420 WP_309174546.1 TIGR03826 family flagellar region protein -
  RE735_RS03165 (RE735_03165) flgM 620417..620680 (+) 264 WP_309174547.1 flagellar biosynthesis anti-sigma factor FlgM -
  RE735_RS03170 (RE735_03170) - 620699..621181 (+) 483 WP_309174548.1 flagellar protein FlgN -
  RE735_RS03175 (RE735_03175) flgK 621197..622717 (+) 1521 WP_309174549.1 flagellar hook-associated protein FlgK -
  RE735_RS03180 (RE735_03180) flgL 622726..623637 (+) 912 WP_309174550.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 52137.86 Da        Isoelectric Point: 9.8951

>NTDB_id=875092 RE735_RS03145 WP_309174543.1 617484..618863(+) (comFA) [Bacillus aerius strain NEB1378]
MNIEKAMELSHKLRSRHLLPSECQCTQSDLEWLEKQGFINRTPAVNRTLSGLICCRCGVSNKRYFALSPCETCQKDCIYC
RSCIMLGKVTECGFLYEWTGPQKKETTQAELTWKGELSKGQQKASDRLLEAIQNKFNLLVWAVCGAGKTEVLFHGIKYAL
NKGMNVCIATPRTDVVLELEPRMRKAFQGLNIVALYGGSPQRFQIAPLMIATTHQLMRYKNAFDVLIVDEVDAFPYSLDE
RLQFAVLKAMTKTGVRIYLSATPSKKMQKDVACGQLEAIKIPLRFHQKPLPVPTFQWVGHWKKKLKKDRLPVSTMNWLQE
HITKKKRVLLFVPSISTMKKVTHILQVQDVNVQGVSADDQERKQKVQHFRDHRYDVLVTTTILERGVTISDVQVGVLGAE
SPIFTESALVQISGRAGRHPEYAKGDVFFFHFGLTKSMKQAKKHIVQMNTAAAQEFSEK

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=875092 RE735_RS03145 WP_309174543.1 617484..618863(+) (comFA) [Bacillus aerius strain NEB1378]
TTGAACATAGAAAAAGCAATGGAACTCAGTCACAAGCTGCGATCAAGACACCTTCTTCCTTCTGAATGCCAATGTACACA
GTCCGATTTGGAGTGGCTGGAGAAGCAAGGGTTTATCAATAGAACACCTGCTGTCAATAGGACGCTGAGTGGTTTGATCT
GCTGCCGATGTGGTGTATCAAACAAGCGCTACTTTGCTCTTTCTCCGTGTGAAACATGTCAAAAAGACTGTATATATTGT
AGATCTTGCATCATGTTGGGAAAAGTAACTGAGTGTGGCTTTCTCTATGAATGGACGGGGCCACAAAAGAAAGAAACAAC
TCAAGCTGAATTAACATGGAAGGGAGAGCTGTCTAAAGGGCAACAGAAAGCGTCCGACAGACTATTAGAAGCAATTCAAA
ACAAATTTAACCTGCTCGTTTGGGCTGTTTGTGGTGCGGGGAAAACGGAGGTGCTTTTTCACGGAATAAAATATGCGCTA
AATAAAGGAATGAATGTTTGTATTGCGACGCCTAGAACCGATGTTGTACTTGAGCTCGAACCGAGAATGAGAAAGGCGTT
TCAAGGACTGAATATCGTGGCACTTTATGGCGGAAGTCCTCAACGATTTCAAATAGCCCCACTTATGATCGCCACAACCC
ATCAGCTGATGAGATATAAAAACGCTTTTGATGTCCTCATTGTAGACGAAGTCGATGCTTTTCCTTATTCCCTTGATGAA
CGGCTTCAGTTTGCTGTTTTAAAGGCAATGACAAAAACAGGAGTTAGAATTTACTTAAGTGCCACACCATCTAAAAAAAT
GCAAAAGGATGTTGCTTGTGGACAATTAGAAGCGATAAAAATTCCTCTGCGTTTCCACCAAAAACCGTTGCCCGTTCCAA
CATTTCAATGGGTTGGACATTGGAAAAAGAAATTAAAAAAGGATCGGCTACCAGTTTCAACAATGAACTGGCTGCAAGAA
CATATTACAAAGAAAAAAAGGGTGTTACTCTTTGTTCCTTCTATTTCTACTATGAAGAAGGTAACTCATATTCTTCAAGT
TCAAGATGTAAATGTACAGGGTGTTTCTGCGGATGATCAAGAAAGGAAGCAAAAAGTCCAGCATTTCAGAGATCATAGAT
ACGATGTATTGGTTACAACAACTATTCTAGAAAGAGGTGTTACCATTTCAGATGTTCAAGTGGGGGTATTAGGTGCGGAA
TCTCCTATTTTTACAGAAAGTGCGCTTGTTCAGATTTCTGGAAGAGCCGGTAGACATCCTGAATATGCTAAAGGAGATGT
TTTCTTTTTTCATTTTGGTTTAACGAAAAGTATGAAACAAGCGAAGAAACATATTGTACAAATGAATACTGCGGCTGCAC
AGGAGTTTTCTGAAAAATAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(116-264)

Helicase conserved C-terminal domain (Helicase_C)

(320-419)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

52.98

98.693

0.523