Detailed information NTG0087384

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6K00_RS13590
• Genome accession: NZ_AP024628
• Coordinates: 2612289..2612906 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain BEST3145
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2607289..2617906

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6K00_RS13560 (BsBEST3145_26320)	rpsT	2607591..2607857 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6K00_RS13565 (BsBEST3145_26330)	holA	2607872..2608915 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6K00_RS13570	-	2608955..2609104 (-)	150	WP_003229985.1	hypothetical protein	-
K6K00_RS13575 (BsBEST3145_26340)	yqzM	2609145..2609279 (+)	135	WP_003229983.1	YqzM family protein	-
K6K00_RS13580	comEC	2609319..2611649 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6K00_RS13585 (BsBEST3145_26370)	comEB	2611653..2612222 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6K00_RS13590 (BsBEST3145_26380)	comEA	2612289..2612906 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6K00_RS13595 (BsBEST3145_26390)	comER	2612990..2613811 (+)	822	WP_004398597.1	late competence protein ComER	-
K6K00_RS13600 (BsBEST3145_26400)	yqeM	2613877..2614620 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6K00_RS13605 (BsBEST3145_26410)	rsfS	2614617..2614973 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6K00_RS13610 (BsBEST3145_26420)	yqeK	2614991..2615551 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6K00_RS13615 (BsBEST3145_26430)	nadD	2615541..2616110 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6K00_RS13620 (BsBEST3145_26440)	yhbY	2616122..2616412 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6K00_RS13625 (BsBEST3145_26450)	aroE	2616406..2617248 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=87384 K6K00_RS13590 WP_004398514.1 2612289..2612906(-) (comEA) [Bacillus subtilis strain BEST3145]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=87384 K6K00_RS13590 WP_004398514.1 2612289..2612906(-) (comEA) [Bacillus subtilis strain BEST3145]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39