Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RCF35_RS16905 Genome accession   NZ_CP133277
Coordinates   3450357..3451427 (-) Length   356 a.a.
NCBI ID   WP_003153083.1    Uniprot ID   -
Organism   Bacillus velezensis strain NBNZ-0060     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3445357..3456427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS16855 (RCF35_16855) sipW 3445416..3446000 (-) 585 WP_032874025.1 signal peptidase I SipW -
  RCF35_RS16860 (RCF35_16860) tapA 3445972..3446643 (-) 672 WP_032874023.1 amyloid fiber anchoring/assembly protein TapA -
  RCF35_RS16865 (RCF35_16865) - 3446902..3447231 (+) 330 WP_032874021.1 DUF3889 domain-containing protein -
  RCF35_RS16870 (RCF35_16870) - 3447272..3447451 (-) 180 WP_022552966.1 YqzE family protein -
  RCF35_RS16875 (RCF35_16875) comGG 3447508..3447885 (-) 378 WP_032874019.1 competence type IV pilus minor pilin ComGG Machinery gene
  RCF35_RS16880 (RCF35_16880) comGF 3447886..3448386 (-) 501 WP_223204419.1 competence type IV pilus minor pilin ComGF -
  RCF35_RS16885 (RCF35_16885) comGE 3448295..3448609 (-) 315 WP_032874016.1 competence type IV pilus minor pilin ComGE Machinery gene
  RCF35_RS16890 (RCF35_16890) comGD 3448593..3449030 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  RCF35_RS16895 (RCF35_16895) comGC 3449020..3449328 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  RCF35_RS16900 (RCF35_16900) comGB 3449333..3450370 (-) 1038 WP_032874014.1 competence type IV pilus assembly protein ComGB Machinery gene
  RCF35_RS16905 (RCF35_16905) comGA 3450357..3451427 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  RCF35_RS16910 (RCF35_16910) - 3451624..3452574 (-) 951 WP_032874012.1 magnesium transporter CorA family protein -
  RCF35_RS16915 (RCF35_16915) - 3452720..3454021 (+) 1302 WP_032874010.1 hemolysin family protein -
  RCF35_RS16920 (RCF35_16920) - 3454059..3454889 (-) 831 WP_032874008.1 STAS domain-containing protein -
  RCF35_RS16930 (RCF35_16930) - 3455095..3455475 (-) 381 WP_032874006.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RCF35_RS16935 (RCF35_16935) - 3455712..3455957 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.85 Da        Isoelectric Point: 9.4729

>NTDB_id=873232 RCF35_RS16905 WP_003153083.1 3450357..3451427(-) (comGA) [Bacillus velezensis strain NBNZ-0060]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=873232 RCF35_RS16905 WP_003153083.1 3450357..3451427(-) (comGA) [Bacillus velezensis strain NBNZ-0060]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCATATACGGCAAGGGCTTCAGATATACACATTGTACC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGGAAGCCGCAAAACGGGTCATTGTCT
TTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAATTGTCCTTATTTCGGGGGGCGGGCGCCGCATTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCCACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAAAGGCATTTCAACCGGCGTATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374