Detailed information NTG0873177

Bioinformatically predicted

Overview

• Name: degS
• Type: Regulator
• Locus tag: RCF35_RS02450
• Genome accession: NZ_CP133277
• Coordinates: 473651..474814 (-)
• Length: 387 a.a.
• NCBI ID: WP_007407484.1
• Uniprot ID: A3KLC1
• Organism: Bacillus velezensis strain NBNZ-0060
• Function: phosphorylation of DegU (predicted from homology) Competence regulation

Genomic Context

Location: 468651..479814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RCF35_RS02420 (RCF35_02420)	-	468915..469334 (-)	420	WP_012118571.1	TIGR03826 family flagellar region protein	-
RCF35_RS02425 (RCF35_02425)	comFC	469408..470097 (-)	690	WP_196770545.1	phosphoribosyltransferase family protein	Machinery gene
RCF35_RS02430 (RCF35_02430)	-	470103..470387 (-)	285	WP_003151389.1	late competence development ComFB family protein	-
RCF35_RS02435 (RCF35_02435)	comFA	470445..471830 (-)	1386	WP_032876163.1	DEAD/DEAH box helicase	Machinery gene
RCF35_RS02440 (RCF35_02440)	-	471939..472787 (-)	849	WP_032876160.1	DegV family protein	-
RCF35_RS02445 (RCF35_02445)	degU	472885..473574 (-)	690	WP_003219701.1	two-component system response regulator DegU	Regulator
RCF35_RS02450 (RCF35_02450)	degS	473651..474814 (-)	1164	WP_007407484.1	two-component sensor histidine kinase DegS	Regulator
RCF35_RS02455 (RCF35_02455)	-	475037..475684 (+)	648	WP_007614107.1	YigZ family protein	-
RCF35_RS02460 (RCF35_02460)	-	475689..476870 (+)	1182	WP_032876157.1	LCP family protein	-
RCF35_RS02465 (RCF35_02465)	-	476969..478045 (-)	1077	WP_003151382.1	glycosyltransferase family 4 protein	-
RCF35_RS02470 (RCF35_02470)	-	478178..479374 (-)	1197	WP_032876154.1	glycosyltransferase	-

Sequence

Protein

• Length: 387 a.a.
• Molecular weight: 45014.78 Da
• Isoelectric Point: 6.8616

>NTDB_id=873177 RCF35_RS02450 WP_007407484.1 473651..474814(-) (degS) [Bacillus velezensis strain NBNZ-0060]
MNKSKMDSKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGDKLEVHTRHARNRLSEVSR
NFHKFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLERRLLGLQEIIERSESLVSQITVVLNYLNQDLRQVGLLL
ADAQAKQDFGLRIIEAQEEERKRVSREIHDGPAQMLANVMMRSELIERIFRDRGTEDGFQEIRNLRQNVRNALYEVRRII
YDLRPMALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGDTENQRLAPQFEVALFRLAQEAVTNALKHSESEEITVKVEV
TADFVVLIIKDNGKGFDIKDAKQKKNKSFGLLGMKERVDLLEGTITIDSKIGLGTFIMIKVPLSLGL

Nucleotide

• Length: 1164 bp

>NTDB_id=873177 RCF35_RS02450 WP_007407484.1 473651..474814(-) (degS) [Bacillus velezensis strain NBNZ-0060]
ATGAATAAATCGAAGATGGACTCCAAAGTTTTGGATTCTATACTTATGAAAATGCTGAAAACCGTGGACGGGAGCAAGGA
CGAGGTTTTTCAAATCGGAGAGCAGTCGCGCCAGCAATATGAGCAGCTGGTTGAAGAACTCAAACAGATCAAACAGCAGG
TGTACGAAGTCATTGAGCTGGGCGACAAACTTGAAGTTCATACGCGGCACGCACGAAACCGCTTGTCTGAAGTAAGCAGA
AATTTCCATAAGTTCAGCGAAGAAGAAATTCGCAACGCCTATGAAAAGGCGCACAAACTTCAAGTGGAACTGACGATGAT
CCAGCAGCGTGAAAAGCAGCTGCGGGAGCGCAGAGATGATTTAGAACGCCGGCTTTTGGGGCTTCAGGAAATTATTGAAC
GGTCAGAATCATTGGTCAGCCAAATAACCGTCGTTCTGAATTACCTGAATCAAGACCTTCGCCAAGTAGGGCTTCTTCTT
GCCGATGCGCAGGCTAAACAAGATTTCGGTTTAAGAATCATTGAAGCCCAAGAGGAAGAAAGAAAAAGAGTTTCCAGAGA
AATTCATGACGGTCCCGCGCAAATGCTGGCAAATGTCATGATGAGATCGGAATTAATTGAACGGATCTTCCGCGACCGCG
GCACGGAGGACGGATTTCAGGAAATCAGAAATCTACGGCAGAATGTGCGGAATGCCCTTTACGAAGTCAGAAGGATTATA
TATGATTTAAGACCGATGGCATTGGACGATCTCGGGCTGATTCCGACACTGAGAAAATATCTTTATACAACTGAAGAATA
CAACGGAAAGGTGAAAATACATTTTCAGTGCATCGGAGACACAGAGAACCAAAGGCTTGCCCCGCAATTTGAAGTGGCGC
TTTTCCGTCTCGCTCAGGAAGCCGTCACAAATGCACTCAAGCATTCAGAATCAGAGGAAATTACAGTAAAAGTGGAAGTA
ACCGCTGATTTTGTCGTTCTCATCATTAAGGACAATGGAAAAGGCTTTGATATTAAGGATGCTAAACAAAAGAAAAACAA
GTCATTCGGCTTGCTGGGAATGAAAGAAAGAGTAGATCTGCTTGAAGGAACGATAACGATAGACTCTAAAATAGGTCTTG
GGACATTTATCATGATTAAGGTTCCGTTATCTCTCGGCCTATAA

Domains

Predicted by InterproScan.

• Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c) (291-384)

• Sensor protein DegS (DegS) (12-170)

• Histidine kinase (HisKA_3) (180-248)

3D structure

• AlphaFold DB: A3KLC1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
degS	Bacillus subtilis subsp. subtilis str. 168	95.584	99.483	0.951