Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   RCF35_RS02435 Genome accession   NZ_CP133277
Coordinates   470445..471830 (-) Length   461 a.a.
NCBI ID   WP_032876163.1    Uniprot ID   -
Organism   Bacillus velezensis strain NBNZ-0060     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 465445..476830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF35_RS02400 (RCF35_02400) flgL 465620..466537 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  RCF35_RS02405 (RCF35_02405) flgK 466549..468066 (-) 1518 WP_032876170.1 flagellar hook-associated protein FlgK -
  RCF35_RS02410 (RCF35_02410) - 468082..468564 (-) 483 WP_032876168.1 flagellar protein FlgN -
  RCF35_RS02415 (RCF35_02415) flgM 468579..468845 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  RCF35_RS02420 (RCF35_02420) - 468915..469334 (-) 420 WP_012118571.1 TIGR03826 family flagellar region protein -
  RCF35_RS02425 (RCF35_02425) comFC 469408..470097 (-) 690 WP_196770545.1 phosphoribosyltransferase family protein Machinery gene
  RCF35_RS02430 (RCF35_02430) - 470103..470387 (-) 285 WP_003151389.1 late competence development ComFB family protein -
  RCF35_RS02435 (RCF35_02435) comFA 470445..471830 (-) 1386 WP_032876163.1 DEAD/DEAH box helicase Machinery gene
  RCF35_RS02440 (RCF35_02440) - 471939..472787 (-) 849 WP_032876160.1 DegV family protein -
  RCF35_RS02445 (RCF35_02445) degU 472885..473574 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  RCF35_RS02450 (RCF35_02450) degS 473651..474814 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  RCF35_RS02455 (RCF35_02455) - 475037..475684 (+) 648 WP_007614107.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52474.86 Da        Isoelectric Point: 9.9925

>NTDB_id=873175 RCF35_RS02435 WP_032876163.1 470445..471830(-) (comFA) [Bacillus velezensis strain NBNZ-0060]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRINGFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWASFAKRQIT
YCRACVMMGRADELTSLYSWNQASENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVVIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
CQKLQLQLPVFLFVPSVHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVIMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=873175 RCF35_RS02435 WP_032876163.1 470445..471830(-) (comFA) [Bacillus velezensis strain NBNZ-0060]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAACGGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGTCCTTTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCTCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTCTGTACGGCGGCAGCGAGGACAAAGGCCGTCTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCGTGATAATAGATGAAGTAGATGCGTTTCCTTTTTCCGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACTGATTGGGTT
TGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTTCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCAGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCATCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATTGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(330-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.826

99.783

0.627
  comFA Latilactobacillus sakei subsp. sakei 23K

38.182

95.445

0.364