Detailed information NTG0087301

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6J86_RS13630
• Genome accession: NZ_AP024627
• Coordinates: 2612726..2613343 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain BEST3136
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2607726..2618343

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6J86_RS13600 (BsBEST3136_26250)	rpsT	2608028..2608294 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6J86_RS13605 (BsBEST3136_26260)	holA	2608309..2609352 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6J86_RS13610	-	2609392..2609541 (-)	150	WP_003229985.1	hypothetical protein	-
K6J86_RS13615 (BsBEST3136_26270)	yqzM	2609582..2609716 (+)	135	WP_003229983.1	YqzM family protein	-
K6J86_RS13620	comEC	2609756..2612086 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6J86_RS13625 (BsBEST3136_26300)	comEB	2612090..2612659 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6J86_RS13630 (BsBEST3136_26310)	comEA	2612726..2613343 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6J86_RS13635 (BsBEST3136_26320)	comER	2613427..2614248 (+)	822	WP_004398597.1	late competence protein ComER	-
K6J86_RS13640 (BsBEST3136_26330)	yqeM	2614314..2615057 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6J86_RS13645 (BsBEST3136_26340)	rsfS	2615054..2615410 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6J86_RS13650 (BsBEST3136_26350)	yqeK	2615428..2615988 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6J86_RS13655 (BsBEST3136_26360)	nadD	2615978..2616547 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6J86_RS13660 (BsBEST3136_26370)	yhbY	2616559..2616849 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6J86_RS13665 (BsBEST3136_26380)	aroE	2616843..2617685 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=87301 K6J86_RS13630 WP_004398514.1 2612726..2613343(-) (comEA) [Bacillus subtilis strain BEST3136]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=87301 K6J86_RS13630 WP_004398514.1 2612726..2613343(-) (comEA) [Bacillus subtilis strain BEST3136]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39