Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   RCF95_RS12660 Genome accession   NZ_CP133197
Coordinates   2102231..2103106 (-) Length   291 a.a.
NCBI ID   WP_308135539.1    Uniprot ID   A0AA51MR15
Organism   Thiothrix subterranea strain DNT53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2097231..2108106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF95_RS12635 - 2097778..2098083 (-) 306 WP_308874976.1 hypothetical protein -
  RCF95_RS12640 - 2098151..2099677 (-) 1527 WP_202716148.1 glucan biosynthesis protein -
  RCF95_RS12645 opgC 2099868..2101049 (+) 1182 WP_308135536.1 OpgC domain-containing protein -
  RCF95_RS12650 ubiE 2100995..2101746 (-) 752 Protein_2484 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  RCF95_RS12655 - 2101828..2102226 (+) 399 WP_308874977.1 hypothetical protein -
  RCF95_RS12660 pilD 2102231..2103106 (-) 876 WP_308135539.1 A24 family peptidase Machinery gene
  RCF95_RS12665 pilC 2103121..2104266 (-) 1146 WP_308872748.1 type II secretion system F family protein Machinery gene
  RCF95_RS12670 pilB 2104464..2106185 (-) 1722 WP_308872749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RCF95_RS12675 - 2106266..2107822 (-) 1557 WP_308135541.1 AAA family ATPase -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32282.65 Da        Isoelectric Point: 7.6369

>NTDB_id=872427 RCF95_RS12660 WP_308135539.1 2102231..2103106(-) (pilD) [Thiothrix subterranea strain DNT53]
MELIFLLKTSPVWLISVVGLFSLLVGSFLNVVIYRLPIMLERQWKQDCNEWLDQPPDKLDTGDFNLVVPRSQCPTCGHKI
TALENIPVISYVFLRGKCAGCKTPISSQYPLVELATALLSMLVAWRVGYGVELVALLGFTWVLVALFMIDAQTMLLPDIL
TYPLLWAGLLLNISGTLVNLPDAVVGAAFGYLALWSVFHLFRLLTGKEGMGHGDFKLLAALGAWGGWQILPFVIFASSAF
GAVFGILWMLAKGKRESLPMPFGPWLAMAGFVAIVWREAILTWMSRIFIPF

Nucleotide


Download         Length: 876 bp        

>NTDB_id=872427 RCF95_RS12660 WP_308135539.1 2102231..2103106(-) (pilD) [Thiothrix subterranea strain DNT53]
ATGGAACTGATTTTTTTACTCAAAACCAGCCCTGTTTGGCTGATAAGCGTCGTGGGGCTATTTTCGCTATTGGTGGGCAG
TTTTCTGAACGTGGTGATCTACCGCCTGCCTATCATGCTGGAACGCCAGTGGAAACAGGATTGCAATGAATGGCTGGATC
AACCACCCGATAAGCTGGATACCGGCGATTTCAATCTGGTCGTGCCACGCTCACAATGCCCGACCTGTGGTCACAAAATC
ACGGCATTGGAAAACATTCCCGTGATCAGCTACGTATTTCTGCGCGGCAAATGTGCCGGTTGCAAAACGCCGATTTCCAG
CCAATATCCGTTGGTGGAATTGGCAACGGCATTGCTGTCCATGTTGGTTGCTTGGCGCGTCGGTTATGGCGTTGAACTGG
TAGCGTTGCTCGGCTTTACTTGGGTCTTGGTCGCGCTGTTTATGATTGACGCGCAAACCATGCTGCTGCCTGACATTCTG
ACGTATCCGCTGCTGTGGGCGGGTTTGCTGCTCAATATCAGTGGCACGTTGGTGAATTTGCCGGATGCGGTGGTGGGCGC
AGCTTTTGGTTATTTGGCGTTGTGGTCAGTGTTTCACCTGTTCCGACTGTTGACGGGCAAGGAAGGCATGGGGCATGGCG
ATTTCAAATTGCTGGCAGCACTCGGCGCGTGGGGTGGCTGGCAAATTCTACCGTTTGTGATTTTTGCGTCATCCGCGTTT
GGGGCGGTGTTTGGCATTTTGTGGATGCTGGCAAAAGGCAAACGCGAAAGCCTGCCCATGCCATTTGGCCCTTGGCTGGC
AATGGCAGGCTTTGTGGCAATCGTATGGCGCGAGGCAATTTTGACGTGGATGAGCCGGATTTTCATCCCCTTCTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(139-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

50.173

99.313

0.498
  pilD Vibrio campbellii strain DS40M4

50.549

93.814

0.474
  pilD Neisseria gonorrhoeae MS11

46.617

91.409

0.426
  pilD Acinetobacter baumannii D1279779

46.591

90.722

0.423
  pilD Acinetobacter nosocomialis M2

46.212

90.722

0.419