Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   RCF95_RS12665 Genome accession   NZ_CP133197
Coordinates   2103121..2104266 (-) Length   381 a.a.
NCBI ID   WP_308872748.1    Uniprot ID   -
Organism   Thiothrix subterranea strain DNT53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2098121..2109266
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCF95_RS12640 - 2098151..2099677 (-) 1527 WP_202716148.1 glucan biosynthesis protein -
  RCF95_RS12645 opgC 2099868..2101049 (+) 1182 WP_308135536.1 OpgC domain-containing protein -
  RCF95_RS12650 ubiE 2100995..2101746 (-) 752 Protein_2484 bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE -
  RCF95_RS12655 - 2101828..2102226 (+) 399 WP_308874977.1 hypothetical protein -
  RCF95_RS12660 pilD 2102231..2103106 (-) 876 WP_308135539.1 A24 family peptidase Machinery gene
  RCF95_RS12665 pilC 2103121..2104266 (-) 1146 WP_308872748.1 type II secretion system F family protein Machinery gene
  RCF95_RS12670 pilB 2104464..2106185 (-) 1722 WP_308872749.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RCF95_RS12675 - 2106266..2107822 (-) 1557 WP_308135541.1 AAA family ATPase -
  RCF95_RS12680 - 2108009..2108833 (+) 825 WP_308872750.1 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 41595.86 Da        Isoelectric Point: 9.9409

>NTDB_id=872428 RCF95_RS12665 WP_308872748.1 2103121..2104266(-) (pilC) [Thiothrix subterranea strain DNT53]
MRGETEAVNPNWLRAELRRKGINPGRIYRKPKPLFKAAIKPGDIAQFARQLTTMMRSGVPMVQSLELMASGGDNASVREL
VNKLRADIEGGASLGSALAKHPKYFDKLFVNLVKAGEQAGTLETMLEKVATYKEKSEALKAKVKSAMMYPIIVVVAAVVV
SAILLIWVIPQFKEVFSSFGADLPAFTLLVISFSDWLVANWWQPILMFVAVGTGFTQARQRSPAFDRFVDKASLKIPIMG
NILNLSAVARFARTLATMFAAGVPLVEAMDSVAGATGNALYEEASRRIQDDTARGVQLNTSMQTTQLFPNMMVQMTRIGE
ESGRLEEMLAKVADYYEAQVDGLVDTLAKQIEPLIMAVLGGIVGSLVIAMYLPIFKLGAVV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=872428 RCF95_RS12665 WP_308872748.1 2103121..2104266(-) (pilC) [Thiothrix subterranea strain DNT53]
ATGCGCGGCGAAACCGAAGCCGTGAACCCCAACTGGTTACGCGCTGAACTGCGACGCAAAGGCATTAATCCGGGGCGTAT
CTACCGCAAACCCAAACCCTTGTTCAAAGCCGCCATCAAACCGGGGGATATTGCGCAATTTGCCCGCCAATTGACCACCA
TGATGCGTTCCGGCGTACCCATGGTGCAATCCTTGGAACTCATGGCTTCCGGGGGCGATAACGCCTCAGTCCGCGAATTG
GTGAACAAATTACGTGCAGACATTGAAGGCGGTGCCAGTTTAGGTTCAGCGTTAGCCAAACACCCCAAGTATTTCGACAA
ACTTTTCGTCAACTTGGTGAAAGCGGGTGAACAGGCCGGTACGCTGGAAACCATGCTGGAAAAAGTCGCCACCTACAAAG
AAAAAAGCGAAGCGCTCAAAGCCAAAGTCAAAAGTGCGATGATGTACCCGATTATCGTCGTGGTTGCTGCCGTGGTGGTA
TCGGCGATTTTGCTGATCTGGGTTATTCCGCAGTTTAAGGAAGTGTTTAGCAGCTTTGGCGCAGACTTGCCTGCTTTCAC
GCTATTGGTCATTTCATTTTCAGATTGGCTGGTCGCTAACTGGTGGCAACCCATTCTGATGTTTGTTGCAGTGGGAACCG
GCTTCACCCAAGCGCGGCAACGCTCGCCAGCCTTTGACCGTTTCGTGGATAAAGCGTCGTTAAAAATCCCGATTATGGGT
AATATCCTGAACTTGTCGGCGGTCGCCCGGTTTGCGCGTACCCTAGCCACCATGTTTGCGGCGGGTGTGCCGTTGGTGGA
AGCGATGGATTCGGTGGCGGGCGCGACCGGCAATGCCTTGTATGAAGAAGCGTCGCGGCGGATTCAAGACGATACCGCTC
GCGGGGTGCAATTGAACACCTCCATGCAAACCACGCAATTATTCCCGAATATGATGGTACAAATGACCCGTATCGGTGAA
GAATCCGGGCGTTTGGAAGAAATGTTGGCAAAAGTAGCCGATTATTACGAAGCACAGGTTGACGGCTTGGTCGACACCTT
GGCAAAACAAATTGAACCGCTGATCATGGCGGTATTGGGCGGTATTGTGGGCAGTTTGGTCATCGCCATGTACTTGCCCA
TCTTTAAACTTGGGGCTGTTGTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(251-373)

Type II secretion system (T2SS), protein F (T2SSF)

(47-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.614

100

0.559
  pilC Acinetobacter baylyi ADP1

50.909

100

0.514
  pilC Acinetobacter baumannii D1279779

49.87

100

0.504
  pilC Legionella pneumophila strain ERS1305867

49.354

100

0.501
  pilG Neisseria gonorrhoeae MS11

44.241

100

0.444
  pilG Neisseria meningitidis 44/76-A

44.241

100

0.444
  pilC Vibrio cholerae strain A1552

39.529

100

0.396
  pilC Vibrio campbellii strain DS40M4

42.733

90.289

0.386
  pilC Thermus thermophilus HB27

40.882

89.239

0.365