Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RB649_RS05195 Genome accession   NZ_CP133179
Coordinates   994566..995147 (+) Length   193 a.a.
NCBI ID   WP_011173434.1    Uniprot ID   Q72IW6
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 989566..1000147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS05165 (RB649_05165) - 989609..989893 (-) 285 WP_226980268.1 hypothetical protein -
  RB649_RS05170 (RB649_05170) - 989775..991091 (+) 1317 WP_338457458.1 HD domain-containing phosphohydrolase -
  RB649_RS05175 (RB649_05175) - 991142..991633 (+) 492 WP_011173430.1 DUF5317 domain-containing protein -
  RB649_RS05180 (RB649_05180) - 991688..992692 (+) 1005 WP_011173431.1 homoisocitrate dehydrogenase -
  RB649_RS05185 (RB649_05185) pilM 992827..993960 (+) 1134 WP_011173432.1 type IV pilus assembly protein PilM Machinery gene
  RB649_RS05190 (RB649_05190) pilN 993953..994576 (+) 624 WP_011173433.1 competence protein Machinery gene
  RB649_RS05195 (RB649_05195) pilO 994566..995147 (+) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  RB649_RS05200 (RB649_05200) pilW 995144..996022 (+) 879 WP_011173435.1 hypothetical protein Machinery gene
  RB649_RS05205 (RB649_05205) pilQ 996019..998292 (+) 2274 WP_011173436.1 secretin N-terminal domain-containing protein Machinery gene
  RB649_RS05210 (RB649_05210) aroC 998351..999502 (+) 1152 WP_011173437.1 chorismate synthase -
  RB649_RS05215 (RB649_05215) - 999527..1000081 (+) 555 WP_011173438.1 shikimate kinase -

Sequence

Protein


Download         Length: 193 a.a.        Molecular weight: 21368.70 Da        Isoelectric Point: 8.4290

>NTDB_id=872287 RB649_RS05195 WP_011173434.1 994566..995147(+) (pilO) [Thermus thermophilus HB27]
MLARLGQREWALLAMVLTALLGLLWYYLLIVPTRQEIATVRQEIDRLTIERNRGLQARRALPELRATIAALQAQRLAFLR
ALPREERLAEVLSEVLQDAEASGVVVRSFTRSRTSAPVPEVRAVNLALALEAPFPETYAYLRRLEDLSRFSTLSGLNLSV
QSQEANPTLATSLTLTVYVLAQGVEAETGGSTP

Nucleotide


Download         Length: 582 bp        

>NTDB_id=872287 RB649_RS05195 WP_011173434.1 994566..995147(+) (pilO) [Thermus thermophilus HB27]
GTGCTCGCTAGGCTGGGACAGCGGGAGTGGGCCCTCCTGGCCATGGTCCTCACCGCCCTCCTGGGGCTCCTCTGGTACTA
CCTGCTCATCGTGCCCACGAGGCAGGAGATCGCCACCGTCCGGCAGGAGATTGACCGCCTCACCATAGAGCGCAACCGCG
GGCTCCAGGCCCGCCGGGCCCTTCCCGAGCTCCGCGCCACCATCGCCGCCCTTCAGGCCCAAAGGCTCGCCTTCCTCCGG
GCCCTGCCCCGGGAGGAAAGGCTCGCCGAGGTCCTCTCCGAGGTGCTCCAGGACGCGGAGGCGAGCGGGGTGGTGGTCCG
AAGCTTCACCCGAAGCCGCACCTCGGCCCCGGTGCCCGAGGTGCGGGCCGTGAACCTGGCCCTGGCCCTCGAGGCCCCCT
TCCCCGAAACCTACGCCTACCTGCGGCGCCTGGAGGACCTCTCCCGCTTCAGCACCCTTTCCGGCCTCAACCTGAGCGTC
CAGAGCCAGGAAGCGAACCCCACCCTCGCCACCAGCCTCACCTTGACCGTCTACGTGCTGGCCCAGGGGGTGGAGGCGGA
AACGGGAGGGAGCACCCCATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(41-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72IW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Thermus thermophilus HB27

100

100

1