Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilW   Type   Machinery gene
Locus tag   RB649_RS05200 Genome accession   NZ_CP133179
Coordinates   995144..996022 (+) Length   292 a.a.
NCBI ID   WP_011173435.1    Uniprot ID   Q72IW5
Organism   Thermus thermophilus HB27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 990144..1001022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS05175 (RB649_05175) - 991142..991633 (+) 492 WP_011173430.1 DUF5317 domain-containing protein -
  RB649_RS05180 (RB649_05180) - 991688..992692 (+) 1005 WP_011173431.1 homoisocitrate dehydrogenase -
  RB649_RS05185 (RB649_05185) pilM 992827..993960 (+) 1134 WP_011173432.1 type IV pilus assembly protein PilM Machinery gene
  RB649_RS05190 (RB649_05190) pilN 993953..994576 (+) 624 WP_011173433.1 competence protein Machinery gene
  RB649_RS05195 (RB649_05195) pilO 994566..995147 (+) 582 WP_011173434.1 type 4a pilus biogenesis protein PilO Machinery gene
  RB649_RS05200 (RB649_05200) pilW 995144..996022 (+) 879 WP_011173435.1 hypothetical protein Machinery gene
  RB649_RS05205 (RB649_05205) pilQ 996019..998292 (+) 2274 WP_011173436.1 secretin N-terminal domain-containing protein Machinery gene
  RB649_RS05210 (RB649_05210) aroC 998351..999502 (+) 1152 WP_011173437.1 chorismate synthase -
  RB649_RS05215 (RB649_05215) - 999527..1000081 (+) 555 WP_011173438.1 shikimate kinase -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 29866.36 Da        Isoelectric Point: 4.6515

>NTDB_id=872288 RB649_RS05200 WP_011173435.1 995144..996022(+) (pilW) [Thermus thermophilus HB27]
MKNAWQRLKEAWANLPRSTKLLLAALLLVGGVALWYVGLYLPAQVAEAPTPAPSTQVIEAPPIPPLASQEEAKPQAEAPA
QEEAQAPASSQEEAQAPPPAPVARAEPPPPNPFVPLVVETPPPPPASASRPTPVPEGPAVRVQTPAQAPQAAPATRPIPG
TSGALPAPKILSPALSAPLPQARETPPRVAVPTALVEAPLPGPEEKGAEKAPTPASPLERLVAEKGLRLSGTLLGPVSVA
ILESKEGYLVVPAGSPIPGTEAVVRQVEEGSVTLALKEETLNLSLVQAGGGQ

Nucleotide


Download         Length: 879 bp        

>NTDB_id=872288 RB649_RS05200 WP_011173435.1 995144..996022(+) (pilW) [Thermus thermophilus HB27]
ATGAAGAACGCCTGGCAACGCCTGAAGGAAGCCTGGGCCAACCTGCCCAGGTCCACCAAGCTCCTCCTGGCGGCCCTCCT
TCTCGTGGGGGGCGTGGCCCTTTGGTACGTGGGCCTCTACCTCCCGGCCCAGGTGGCGGAAGCGCCCACCCCGGCCCCCT
CCACGCAGGTCATCGAGGCCCCCCCGATCCCGCCCCTCGCCTCCCAGGAAGAGGCCAAGCCACAGGCCGAAGCCCCGGCC
CAAGAGGAGGCCCAGGCGCCCGCTTCTTCCCAAGAGGAGGCCCAGGCGCCCCCGCCTGCCCCCGTGGCCCGGGCCGAGCC
CCCGCCCCCGAACCCCTTCGTCCCCCTGGTGGTGGAAACCCCACCCCCACCTCCGGCCTCCGCCTCCCGCCCCACGCCCG
TCCCCGAGGGGCCCGCCGTCCGGGTCCAGACCCCCGCCCAAGCCCCCCAGGCGGCCCCCGCCACCCGGCCCATCCCCGGG
ACCTCCGGCGCCCTCCCGGCGCCCAAGATCCTCTCCCCCGCGCTGTCCGCCCCCCTGCCCCAGGCCAGGGAGACGCCGCC
TAGGGTCGCCGTGCCCACGGCGCTCGTGGAGGCCCCCCTCCCCGGGCCCGAGGAAAAGGGGGCGGAGAAGGCCCCCACCC
CGGCCTCGCCGCTGGAGCGCCTGGTGGCGGAAAAGGGCCTCCGCCTCTCGGGGACCCTGCTCGGCCCCGTGAGCGTGGCC
ATCCTGGAGAGCAAGGAGGGCTACCTCGTCGTCCCGGCGGGAAGCCCCATCCCCGGCACGGAGGCCGTGGTGCGCCAGGT
GGAGGAAGGAAGCGTGACCCTGGCCTTGAAGGAGGAAACCTTGAACCTGTCCCTCGTCCAAGCTGGAGGTGGCCAATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72IW5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilW Thermus thermophilus HB27

100

100

1