Detailed information NTG0872285

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RB649_RS05185
• Genome accession: NZ_CP133179
• Coordinates: 992827..993960 (+)
• Length: 377 a.a.
• NCBI ID: WP_011173432.1
• Uniprot ID: -
• Organism: Thermus thermophilus HB27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 987827..998960

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RB649_RS05145 (RB649_05145)	-	987828..988586 (+)	759	WP_011173427.1	type III pantothenate kinase	-
RB649_RS05150 (RB649_05150)	-	988549..988914 (-)	366	WP_024119691.1	hypothetical protein	-
RB649_RS05165 (RB649_05165)	-	989609..989893 (-)	285	WP_226980268.1	hypothetical protein	-
RB649_RS05170 (RB649_05170)	-	989775..991091 (+)	1317	WP_338457458.1	HD domain-containing phosphohydrolase	-
RB649_RS05175 (RB649_05175)	-	991142..991633 (+)	492	WP_011173430.1	DUF5317 domain-containing protein	-
RB649_RS05180 (RB649_05180)	-	991688..992692 (+)	1005	WP_011173431.1	homoisocitrate dehydrogenase	-
RB649_RS05185 (RB649_05185)	pilM	992827..993960 (+)	1134	WP_011173432.1	type IV pilus assembly protein PilM	Machinery gene
RB649_RS05190 (RB649_05190)	pilN	993953..994576 (+)	624	WP_011173433.1	competence protein	Machinery gene
RB649_RS05195 (RB649_05195)	pilO	994566..995147 (+)	582	WP_011173434.1	type 4a pilus biogenesis protein PilO	Machinery gene
RB649_RS05200 (RB649_05200)	pilW	995144..996022 (+)	879	WP_011173435.1	hypothetical protein	Machinery gene
RB649_RS05205 (RB649_05205)	pilQ	996019..998292 (+)	2274	WP_011173436.1	secretin N-terminal domain-containing protein	Machinery gene

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 41399.02 Da
• Isoelectric Point: 4.3805

>NTDB_id=872285 RB649_RS05185 WP_011173432.1 992827..993960(+) (pilM) [Thermus thermophilus HB27]
MFKSLSQLFRPRVEALGLEIGASALKLVEVSGNPPALKALASRPTPPGLLMEGMVAEPAALAQEIKELLLEARTRKRYVV
TALSNLAVILRPIQVPKMPLKEMEEAVRWEAERYIPFPIDEVVLDFAPLTPLSEVQEGEQVQVMVAAARQEAVAGVLEAL
RGAGLVPVVLDVKPFAGLYPLEARLAEEPDRVFLVLDIGAESTSLVLLRGDKPLAVRVLTLSGKDFTEAIARSFNLDLLA
AEEVKRTYGMATLPTEDEELLLDFDAERERYSPGRIYDAIRPVLVELTQELRRSLEFFRIQLEEASPEMGYLLGGGSKLR
GLASLLTDTLGVNFEPVNPWEAVAVDPKRFESEQLQEIGPEFAVALGLALRGVEPLD

Nucleotide

• Length: 1134 bp

>NTDB_id=872285 RB649_RS05185 WP_011173432.1 992827..993960(+) (pilM) [Thermus thermophilus HB27]
GTGTTCAAAAGCCTTAGCCAGCTCTTCCGACCCAGGGTTGAGGCCCTGGGCTTGGAGATCGGGGCCTCCGCCCTGAAGCT
CGTGGAGGTGTCCGGGAACCCCCCTGCCCTCAAAGCCCTGGCCTCCCGCCCCACCCCGCCCGGCCTCCTGATGGAGGGAA
TGGTGGCCGAGCCTGCCGCGCTGGCCCAGGAGATCAAGGAGCTTCTCCTCGAGGCCCGCACCCGCAAGCGCTACGTGGTC
ACCGCCCTCTCCAACCTGGCGGTGATCCTCCGCCCCATCCAGGTTCCCAAGATGCCCCTCAAGGAGATGGAGGAGGCGGT
GCGCTGGGAGGCGGAGCGCTACATTCCCTTCCCCATTGACGAGGTGGTCCTGGACTTCGCCCCCCTGACCCCCCTCTCCG
AGGTGCAGGAGGGGGAGCAGGTCCAGGTGATGGTGGCGGCGGCGCGGCAGGAGGCGGTGGCGGGGGTCCTCGAGGCCCTG
AGGGGGGCGGGGCTCGTCCCCGTGGTGCTGGACGTGAAGCCCTTCGCCGGGCTTTACCCTCTGGAGGCCAGGCTTGCGGA
GGAGCCCGACCGGGTCTTCCTCGTCCTGGACATCGGGGCCGAGAGCACGAGCCTCGTCCTGCTCCGGGGGGACAAGCCCC
TGGCGGTGCGCGTCCTCACCCTCTCGGGCAAGGACTTCACCGAGGCCATCGCCCGAAGCTTCAACCTGGACCTCCTCGCC
GCCGAGGAGGTGAAGCGGACCTACGGGATGGCCACCCTCCCCACCGAGGACGAGGAGCTCCTCCTGGACTTTGACGCCGA
GCGGGAGCGCTACAGCCCGGGGCGCATCTACGACGCCATCCGCCCGGTGCTGGTGGAGCTCACCCAGGAGCTCCGCCGTA
GCCTGGAGTTCTTCCGCATCCAGCTGGAGGAGGCCTCGCCCGAGATGGGCTACCTCCTGGGCGGGGGGAGCAAGCTCAGG
GGGCTCGCCTCCCTCCTCACCGACACCCTGGGGGTGAACTTTGAGCCCGTCAACCCCTGGGAGGCGGTGGCGGTGGACCC
CAAGCGCTTTGAGTCGGAGCAACTCCAGGAGATCGGGCCGGAGTTCGCCGTGGCCCTGGGCTTGGCGTTGAGGGGGGTGG
AGCCCCTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (17-372)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	100	100	1