Detailed information    

insolico Bioinformatically predicted

Overview

Name   comA   Type   Machinery gene
Locus tag   RAM80_RS25295 Genome accession   NZ_CP133165
Coordinates   5492309..5494531 (+) Length   740 a.a.
NCBI ID   WP_416423131.1    Uniprot ID   -
Organism   Pseudomonas sp. App30     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5487309..5499531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM80_RS25275 (RAM80_25195) - 5489079..5489702 (+) 624 WP_416423127.1 glutathione S-transferase -
  RAM80_RS25280 (RAM80_25200) - 5489846..5490778 (+) 933 WP_416423128.1 ABC transporter ATP-binding protein -
  RAM80_RS25285 (RAM80_25205) - 5490775..5491554 (+) 780 WP_416423129.1 ABC transporter permease -
  RAM80_RS25290 (RAM80_25210) - 5491649..5492161 (-) 513 WP_416423130.1 DUF2062 domain-containing protein -
  RAM80_RS25295 (RAM80_25215) comA 5492309..5494531 (+) 2223 WP_416423131.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RAM80_RS25300 (RAM80_25220) exbB 5494594..5495229 (+) 636 WP_416423132.1 MotA/TolQ/ExbB proton channel family protein Machinery gene
  RAM80_RS25305 (RAM80_25225) - 5495226..5495651 (+) 426 WP_416423133.1 ExbD/TolR family protein -
  RAM80_RS25310 (RAM80_25230) lpxK 5495651..5496652 (+) 1002 WP_416423134.1 tetraacyldisaccharide 4'-kinase -
  RAM80_RS25315 (RAM80_25235) - 5496694..5496879 (+) 186 WP_027977460.1 Trm112 family protein -
  RAM80_RS25320 (RAM80_25240) kdsB 5496876..5497640 (+) 765 WP_416423135.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  RAM80_RS25325 (RAM80_25245) - 5497640..5498104 (+) 465 WP_416423136.1 low molecular weight protein-tyrosine-phosphatase -
  RAM80_RS25330 (RAM80_25250) murB 5498101..5499120 (+) 1020 WP_416423137.1 UDP-N-acetylmuramate dehydrogenase -

Sequence

Protein


Download         Length: 740 a.a.        Molecular weight: 79454.39 Da        Isoelectric Point: 11.4812

>NTDB_id=872268 RAM80_RS25295 WP_416423131.1 5492309..5494531(+) (comA) [Pseudomonas sp. App30]
MRTAMLALATGLVALRFLPVLPGTPLLLGLVALGMALCWWRWARLGLLVLGLAWAAWQGQRALDDRLSLALDGQTLWLEG
RVSGLPDNQGASVRFEVSDPQARRHALPATVRLSWFNGPVVNAGERWRLAVTLKRPRGLVNPAGLDAEAWMLARRIGATG
SVKDGQLQAPAQGAWRDGLRQRLLAVDAQGYQGVLVALVMGDGSAISREQWNVLQATGTVHLLVISGTHIGLLAGLVYGL
VAALARFGLWPRALPWLPWACGLAFAAALGYGLLAGFQVPVRRACIMLGLVLLWRLRFRHLGVWLPLLVALNAVLLSDPL
VSLLPGFWLSFAAVAVLMLVFSGRLGAWPWWRAWTRAQWLMAVGLLPAMLALGLPVSLGGPLANLLAVPWVSLAVLPTAL
LGALLLPLPWVGEGLLWLAGGLLAVLFDALGWLAARVPAWLPPALPLGAWLLVLAGTLLLLLPAGMPLRPLGWPLVLLAA
LPPQASVPHGQAQVTQLDVGQGLAVLVRTRNHALLYDTGPRTPGQDAGERVVLPNLHGLGVRRLDLMLISHAHMDHAGGA
PAVMRGVPVAAVWSGEAGQLPAQLGAQPCEAGHQWQWDGVQFTVWRWAAARESNPSSCVLLVEANGEKLLLGGDIDAAAE
RALVASGLDLAGHWLQSPHHGSRTSSSQLLLTAMKPRGALISRGHNNSFGHPHPLVVERYRANGIEVHDNAVQGALHLRL
GRFDGVRGERERRRFWRDPT

Nucleotide


Download         Length: 2223 bp        

>NTDB_id=872268 RAM80_RS25295 WP_416423131.1 5492309..5494531(+) (comA) [Pseudomonas sp. App30]
ATGCGTACTGCGATGCTGGCACTGGCCACGGGCCTGGTGGCGCTGCGTTTTCTCCCGGTGCTGCCAGGCACCCCGCTGTT
GCTGGGGCTCGTGGCGCTGGGCATGGCGCTGTGCTGGTGGCGTTGGGCGCGCCTGGGGCTGCTGGTGCTGGGCCTGGCCT
GGGCGGCCTGGCAAGGGCAGCGTGCGCTGGATGACCGTCTGTCGCTCGCCCTGGATGGCCAGACCCTGTGGCTGGAGGGG
CGAGTCAGCGGGCTGCCGGACAACCAGGGCGCCTCCGTGCGTTTCGAGGTGAGCGACCCGCAGGCGCGGCGCCATGCCCT
GCCAGCCACGGTGCGCCTGTCGTGGTTCAACGGGCCGGTGGTCAATGCCGGTGAGCGCTGGCGCCTGGCGGTGACGTTGA
AGCGGCCGCGGGGGCTGGTGAACCCGGCGGGCCTGGATGCCGAAGCCTGGATGCTGGCCCGGCGCATCGGCGCCACCGGC
AGCGTCAAGGACGGCCAGCTGCAGGCCCCGGCGCAAGGAGCCTGGCGCGACGGCCTGCGCCAACGCCTGCTGGCGGTGGA
TGCCCAGGGCTACCAGGGCGTGCTGGTGGCGCTGGTGATGGGCGACGGTTCGGCCATCAGTCGCGAGCAATGGAACGTGC
TGCAAGCCACTGGCACCGTGCACCTGCTGGTGATTTCCGGCACGCACATCGGCCTGCTGGCCGGCCTGGTCTACGGCCTC
GTGGCAGCGCTGGCGCGTTTCGGGCTGTGGCCGCGGGCGCTGCCCTGGCTGCCCTGGGCGTGTGGCCTGGCCTTCGCCGC
GGCGCTGGGCTACGGGCTGCTGGCCGGGTTCCAGGTGCCGGTGCGACGGGCGTGCATCATGCTCGGGCTGGTGCTGTTGT
GGCGCCTGCGCTTCCGCCATCTGGGGGTGTGGCTGCCGCTGCTGGTGGCGTTGAACGCGGTGTTGCTGAGCGACCCACTG
GTGAGCCTGCTGCCTGGGTTCTGGCTGTCGTTCGCGGCGGTGGCGGTGTTGATGCTGGTGTTTTCCGGCAGGCTGGGTGC
CTGGCCGTGGTGGCGCGCCTGGACCCGCGCGCAATGGCTGATGGCCGTGGGTTTGCTGCCGGCGATGCTGGCGCTGGGGT
TGCCCGTGAGCCTCGGCGGCCCGCTGGCCAATCTGCTGGCGGTGCCGTGGGTGAGCCTGGCGGTGTTGCCCACGGCGCTG
CTGGGCGCCCTGTTGCTGCCGCTGCCGTGGGTGGGCGAAGGGCTGCTGTGGCTGGCGGGCGGCCTGCTGGCCGTGTTGTT
CGACGCCCTGGGCTGGTTGGCGGCGCGGGTGCCGGCGTGGCTGCCGCCGGCGTTGCCCCTGGGCGCGTGGCTGCTGGTGC
TGGCCGGCACGTTGCTGCTGTTGCTGCCAGCTGGCATGCCCTTGCGTCCGCTGGGCTGGCCGCTGGTGCTGCTGGCGGCG
CTGCCGCCCCAGGCGTCAGTGCCCCATGGCCAGGCCCAGGTGACCCAGCTGGACGTGGGGCAAGGCCTGGCGGTGCTGGT
GCGTACGCGCAATCACGCACTGTTGTACGACACCGGCCCGCGCACGCCGGGGCAGGATGCCGGTGAGCGGGTGGTGTTGC
CCAACCTGCATGGCCTGGGCGTGCGGCGCCTGGACCTGATGCTGATCAGCCACGCGCACATGGACCACGCCGGTGGCGCC
CCGGCGGTGATGCGCGGGGTGCCGGTGGCCGCAGTGTGGAGCGGCGAGGCTGGGCAATTGCCGGCGCAGCTGGGCGCGCA
GCCCTGCGAGGCCGGGCACCAGTGGCAGTGGGATGGGGTGCAATTCACCGTGTGGCGCTGGGCGGCGGCGCGGGAGAGCA
ATCCGTCGTCCTGCGTGCTGCTGGTGGAGGCGAATGGCGAGAAGCTGCTGTTGGGTGGGGATATCGACGCCGCTGCCGAG
CGTGCGTTGGTGGCCAGTGGCCTGGACCTGGCCGGGCACTGGTTGCAGTCGCCCCACCACGGCAGCCGCACCTCGTCATC
GCAATTGCTGCTGACGGCCATGAAACCGCGCGGCGCGCTGATTTCCCGCGGCCATAACAACTCGTTCGGGCATCCGCACC
CGCTGGTGGTCGAGCGTTACCGCGCCAACGGCATCGAAGTCCACGACAACGCCGTGCAGGGTGCGCTGCACCTGCGTCTG
GGGCGCTTCGACGGGGTGCGTGGCGAGCGCGAGCGGCGGCGCTTCTGGCGTGACCCGACATGA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(498-685)

Competence protein (Competence)

(198-462)

Domain of unknown function (DUF4131) (DUF4131)

(26-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comA Pseudomonas stutzeri DSM 10701

60.64

97.162

0.589
  comA Ralstonia pseudosolanacearum GMI1000

36.514

100

0.388