Detailed information NTG0087218

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6K02_RS13605
• Genome accession: NZ_AP024626
• Coordinates: 2606345..2606962 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain BEST3125
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2601345..2611962

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6K02_RS13575 (BsBEST3125_26060)	rpsT	2601647..2601913 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6K02_RS13580 (BsBEST3125_26070)	holA	2601928..2602971 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6K02_RS13585	-	2603011..2603160 (-)	150	WP_003229985.1	hypothetical protein	-
K6K02_RS13590 (BsBEST3125_26080)	yqzM	2603201..2603335 (+)	135	WP_003229983.1	YqzM family protein	-
K6K02_RS13595	comEC	2603375..2605705 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6K02_RS13600 (BsBEST3125_26110)	comEB	2605709..2606278 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6K02_RS13605 (BsBEST3125_26120)	comEA	2606345..2606962 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6K02_RS13610 (BsBEST3125_26130)	comER	2607046..2607867 (+)	822	WP_004398597.1	late competence protein ComER	-
K6K02_RS13615 (BsBEST3125_26140)	yqeM	2607933..2608676 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6K02_RS13620 (BsBEST3125_26150)	rsfS	2608673..2609029 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6K02_RS13625 (BsBEST3125_26160)	yqeK	2609047..2609607 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6K02_RS13630 (BsBEST3125_26170)	nadD	2609597..2610166 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6K02_RS13635 (BsBEST3125_26180)	yhbY	2610178..2610468 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6K02_RS13640 (BsBEST3125_26190)	aroE	2610462..2611304 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=87218 K6K02_RS13605 WP_004398514.1 2606345..2606962(-) (comEA) [Bacillus subtilis strain BEST3125]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=87218 K6K02_RS13605 WP_004398514.1 2606345..2606962(-) (comEA) [Bacillus subtilis strain BEST3125]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39