Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   BsBEST3145_RS18885 Genome accession   NZ_AP024625
Coordinates   3608244..3609635 (-) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis strain BEST3109     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3603244..3614635
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BsBEST3145_RS18850 (BsBEST3109_36290) flgL 3603415..3604311 (-) 897 WP_003228004.1 flagellar hook-associated protein FlgL -
  BsBEST3145_RS18855 (BsBEST3109_36300) flgK 3604322..3605845 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  BsBEST3145_RS18860 (BsBEST3109_36310) flgN 3605864..3606346 (-) 483 WP_003227999.1 flagellar protein FlgN -
  BsBEST3145_RS18865 (BsBEST3109_36320) flgM 3606362..3606628 (-) 267 WP_003227997.1 flagellar biosynthesis anti-sigma factor FlgM -
  BsBEST3145_RS18870 (BsBEST3109_36330) yvyF 3606709..3607128 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  BsBEST3145_RS18875 (BsBEST3109_36340) comFC 3607202..3607924 (-) 723 WP_003227991.1 comF operon protein ComFC Machinery gene
  BsBEST3145_RS18880 (BsBEST3109_36350) comFB 3607888..3608184 (-) 297 WP_003227989.1 late competence protein ComFB -
  BsBEST3145_RS18885 (BsBEST3109_36360) comFA 3608244..3609635 (-) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  BsBEST3145_RS18890 (BsBEST3109_36370) fakBA 3609741..3610586 (-) 846 WP_003244125.1 DegV family protein -
  BsBEST3145_RS18895 (BsBEST3109_36380) degU 3610684..3611373 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  BsBEST3145_RS18900 (BsBEST3109_36390) degS 3611456..3612613 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  BsBEST3145_RS18905 (BsBEST3109_36400) yvyE 3612830..3613483 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=87150 BsBEST3145_RS18885 WP_003243962.1 3608244..3609635(-) (comFA) [Bacillus subtilis strain BEST3109]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=87150 BsBEST3145_RS18885 WP_003243962.1 3608244..3609635(-) (comFA) [Bacillus subtilis strain BEST3109]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1

Multiple sequence alignment