Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   RA292_RS12295 Genome accession   NZ_CP132912
Coordinates   2414014..2414631 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis subsp. natto strain BN-P15-11-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2409014..2419631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA292_RS12265 rpsT 2409316..2409582 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  RA292_RS12270 holA 2409597..2410640 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  RA292_RS12275 - 2410680..2410829 (-) 150 WP_003229985.1 hypothetical protein -
  RA292_RS12280 yqzM 2410870..2411004 (+) 135 WP_003229983.1 YqzM family protein -
  RA292_RS12285 comEC 2411044..2413374 (-) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  RA292_RS12290 comEB 2413378..2413947 (-) 570 WP_003229978.1 ComE operon protein 2 -
  RA292_RS12295 comEA 2414014..2414631 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  RA292_RS12300 comER 2414715..2415536 (+) 822 WP_004398597.1 late competence protein ComER -
  RA292_RS12305 yqeM 2415602..2416345 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  RA292_RS12310 rsfS 2416342..2416698 (-) 357 WP_003229971.1 ribosome silencing factor -
  RA292_RS12315 yqeK 2416716..2417276 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  RA292_RS12320 nadD 2417266..2417835 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  RA292_RS12325 yhbY 2417847..2418137 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  RA292_RS12330 aroE 2418131..2418973 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=870660 RA292_RS12295 WP_004398514.1 2414014..2414631(-) (comEA) [Bacillus subtilis subsp. natto strain BN-P15-11-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=870660 RA292_RS12295 WP_004398514.1 2414014..2414631(-) (comEA) [Bacillus subtilis subsp. natto strain BN-P15-11-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39