Detailed information NTG0087046

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6J91_RS13640
• Genome accession: NZ_AP024624
• Coordinates: 2606327..2606944 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain BEST3106
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2601327..2611944

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6J91_RS13610 (BsBEST3106_26060)	rpsT	2601629..2601895 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6J91_RS13615 (BsBEST3106_26070)	holA	2601910..2602953 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6J91_RS13620	-	2602993..2603142 (-)	150	WP_003229985.1	hypothetical protein	-
K6J91_RS13625 (BsBEST3106_26080)	yqzM	2603183..2603317 (+)	135	WP_003229983.1	YqzM family protein	-
K6J91_RS13630	comEC	2603357..2605687 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6J91_RS13635 (BsBEST3106_26110)	comEB	2605691..2606260 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6J91_RS13640 (BsBEST3106_26120)	comEA	2606327..2606944 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6J91_RS13645 (BsBEST3106_26130)	comER	2607028..2607849 (+)	822	WP_004398597.1	late competence protein ComER	-
K6J91_RS13650 (BsBEST3106_26140)	yqeM	2607915..2608658 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6J91_RS13655 (BsBEST3106_26150)	rsfS	2608655..2609011 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6J91_RS13660 (BsBEST3106_26160)	yqeK	2609029..2609589 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6J91_RS13665 (BsBEST3106_26170)	nadD	2609579..2610148 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6J91_RS13670 (BsBEST3106_26180)	yhbY	2610160..2610450 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6J91_RS13675 (BsBEST3106_26190)	aroE	2610444..2611286 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=87046 K6J91_RS13640 WP_004398514.1 2606327..2606944(-) (comEA) [Bacillus subtilis strain BEST3106]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=87046 K6J91_RS13640 WP_004398514.1 2606327..2606944(-) (comEA) [Bacillus subtilis strain BEST3106]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39