Detailed information NTG0869612

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: RA177_RS16365
• Genome accession: NZ_CP132599
• Coordinates: 3204217..3205614 (-)
• Length: 465 a.a.
• NCBI ID: WP_306655275.1
• Uniprot ID: -
• Organism: Bacillus safensis strain PL23A
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3199217..3210614

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RA177_RS16330 (RA177_16345)	flgL	3199417..3200325 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
RA177_RS16335 (RA177_16350)	flgK	3200335..3201855 (-)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
RA177_RS16340 (RA177_16355)	-	3201871..3202353 (-)	483	WP_034623751.1	flagellar protein FlgN	-
RA177_RS16345 (RA177_16360)	flgM	3202372..3202635 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
RA177_RS16350 (RA177_16365)	-	3202715..3203134 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
RA177_RS16355 (RA177_16370)	comFC	3203192..3203848 (-)	657	WP_231506847.1	ComF family protein	Machinery gene
RA177_RS16360 (RA177_16375)	-	3203875..3204168 (-)	294	WP_024426256.1	late competence development ComFB family protein	-
RA177_RS16365 (RA177_16380)	comFA	3204217..3205614 (-)	1398	WP_306655275.1	DEAD/DEAH box helicase	Machinery gene
RA177_RS16370 (RA177_16385)	-	3205735..3206577 (-)	843	WP_024423625.1	DegV family protein	-
RA177_RS16375 (RA177_16390)	degU	3206793..3207482 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
RA177_RS16380 (RA177_16395)	degS	3207547..3208722 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator
RA177_RS16385 (RA177_16400)	-	3208943..3209611 (+)	669	WP_012011434.1	YigZ family protein	-

Sequence

Protein

• Length: 465 a.a.
• Molecular weight: 53166.12 Da
• Isoelectric Point: 10.0163

>NTDB_id=869612 RA177_RS16365 WP_306655275.1 3204217..3205614(-) (comFA) [Bacillus safensis strain PL23A]
MNMDKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC
RSCIMMGKAAECGFLYEWAGPQMVETCRAELTWQGELSKGQKRASERMVEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMSVCIATPRTDVVLELEPRLRKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPIPTFHWIGHWKKKLKKNQLPPKVMNWMQ
RHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPNRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKRCGFN

Nucleotide

• Length: 1398 bp

>NTDB_id=869612 RA177_RS16365 WP_306655275.1 3204217..3205614(-) (comFA) [Bacillus safensis strain PL23A]
TTGAATATGGACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGCCATCTGCTCACTGTTGAAACAAAGTGTCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAGGTGTGTCAAAAGGATTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTTTATGAATGGGCAGGTCCACAAATGGTAGAAACATG
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGGTTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCGTTA
AATCAAGGAATGAGTGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTAGAAAAGCATT
TCAAGGGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCATAC
CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG
AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG
AGAACAATCCTTGAACGTGGAGGGGGTATCTGCCGATGATCCGAATAGGAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTCCAGATATCTGGACGGGTAGGCAGACACCCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACGGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (324-420)

• Type III restriction enzyme, res subunit (ResIII) (116-265)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.974	98.065	0.51
comFA/cflA	Streptococcus pneumoniae Rx1	38.095	94.839	0.361
comFA/cflA	Streptococcus pneumoniae D39	38.095	94.839	0.361
comFA/cflA	Streptococcus pneumoniae R6	38.095	94.839	0.361