Detailed information NTG0086960

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6J99_RS13630
• Genome accession: NZ_AP024623
• Coordinates: 2609112..2609729 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain BEST3102
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2604112..2614729

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6J99_RS13600 (BsBEST3102_26040)	rpsT	2604414..2604680 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6J99_RS13605 (BsBEST3102_26050)	holA	2604695..2605738 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6J99_RS13610	-	2605778..2605927 (-)	150	WP_003229985.1	hypothetical protein	-
K6J99_RS13615 (BsBEST3102_26060)	yqzM	2605968..2606102 (+)	135	WP_003229983.1	YqzM family protein	-
K6J99_RS13620	comEC	2606142..2608472 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6J99_RS13625 (BsBEST3102_26090)	comEB	2608476..2609045 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6J99_RS13630 (BsBEST3102_26100)	comEA	2609112..2609729 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6J99_RS13635 (BsBEST3102_26110)	comER	2609813..2610634 (+)	822	WP_004398597.1	late competence protein ComER	-
K6J99_RS13640 (BsBEST3102_26120)	yqeM	2610700..2611443 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6J99_RS13645 (BsBEST3102_26130)	rsfS	2611440..2611796 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6J99_RS13650 (BsBEST3102_26140)	yqeK	2611814..2612374 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6J99_RS13655 (BsBEST3102_26150)	nadD	2612364..2612933 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6J99_RS13660 (BsBEST3102_26160)	yhbY	2612945..2613235 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6J99_RS13665 (BsBEST3102_26170)	aroE	2613229..2614071 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=86960 K6J99_RS13630 WP_004398514.1 2609112..2609729(-) (comEA) [Bacillus subtilis strain BEST3102]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=86960 K6J99_RS13630 WP_004398514.1 2609112..2609729(-) (comEA) [Bacillus subtilis strain BEST3102]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39