Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC   Type   Machinery gene
Locus tag   RA177_RS11385 Genome accession   NZ_CP132599
Coordinates   2258025..2258318 (-) Length   97 a.a.
NCBI ID   WP_024422977.1    Uniprot ID   A0A0M2EIN6
Organism   Bacillus safensis strain PL23A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2253025..2263318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RA177_RS11335 (RA177_11350) sinR 2253192..2253527 (+) 336 WP_024422969.1 transcriptional regulator SinR Regulator
  RA177_RS11340 (RA177_11355) tasA 2253595..2254395 (-) 801 WP_024422970.1 biofilm matrix protein TasA -
  RA177_RS11345 (RA177_11360) sipW 2254450..2255022 (-) 573 WP_024422971.1 signal peptidase I SipW -
  RA177_RS11350 (RA177_11365) tapA 2255075..2255602 (-) 528 WP_103131952.1 amyloid fiber anchoring/assembly protein TapA -
  RA177_RS11355 (RA177_11370) - 2255902..2256180 (+) 279 WP_171464609.1 DUF3889 domain-containing protein -
  RA177_RS11360 (RA177_11375) - 2256215..2256409 (-) 195 WP_024422974.1 YqzE family protein -
  RA177_RS11365 (RA177_11380) - 2256469..2256852 (-) 384 WP_103131951.1 competence type IV pilus minor pilin ComGG -
  RA177_RS11370 (RA177_11385) comGF 2256849..2257376 (-) 528 WP_258011119.1 competence type IV pilus minor pilin ComGF -
  RA177_RS11375 (RA177_11390) comGE 2257288..2257602 (-) 315 WP_073206282.1 competence type IV pilus minor pilin ComGE -
  RA177_RS11380 (RA177_11395) comGD 2257586..2258032 (-) 447 WP_185853687.1 competence type IV pilus minor pilin ComGD -
  RA177_RS11385 (RA177_11400) comGC 2258025..2258318 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  RA177_RS11390 (RA177_11405) comGB 2258335..2259375 (-) 1041 WP_103131948.1 competence type IV pilus assembly protein ComGB -
  RA177_RS11395 (RA177_11410) comGA 2259356..2260426 (-) 1071 WP_025092972.1 competence type IV pilus ATPase ComGA Machinery gene
  RA177_RS11400 (RA177_11415) - 2260604..2261479 (-) 876 WP_024422979.1 STAS domain-containing protein -
  RA177_RS11410 (RA177_11425) - 2261703..2262083 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  RA177_RS11415 (RA177_11430) - 2262296..2262538 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  RA177_RS11420 (RA177_11435) - 2262586..2263224 (-) 639 WP_024422981.1 MBL fold metallo-hydrolase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 10686.62 Da        Isoelectric Point: 7.3218

>NTDB_id=869594 RA177_RS11385 WP_024422977.1 2258025..2258318(-) (comGC) [Bacillus safensis strain PL23A]
MNDKGFTLLEMLIVMLVISILLLITIPNITRHHQAIQAKGCEGLKSMVSTQVMAYELEHDGKTPSLAELEKEGYVKKGLT
CPNGKAIVIQNGNVKHE

Nucleotide


Download         Length: 294 bp        

>NTDB_id=869594 RA177_RS11385 WP_024422977.1 2258025..2258318(-) (comGC) [Bacillus safensis strain PL23A]
ATGAATGATAAAGGATTTACCCTGTTAGAAATGTTAATAGTTATGCTCGTAATATCCATTCTTTTACTCATTACCATACC
GAATATCACACGTCATCACCAAGCGATTCAGGCAAAGGGCTGTGAAGGGCTTAAATCAATGGTCAGCACACAAGTGATGG
CATATGAATTGGAGCATGATGGGAAGACGCCATCTTTAGCTGAATTAGAAAAAGAAGGTTATGTCAAAAAGGGGTTAACT
TGTCCTAATGGAAAAGCGATTGTCATTCAAAACGGCAACGTGAAGCATGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0M2EIN6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Bacillus subtilis subsp. subtilis str. 168

67.01

100

0.67
  comGC Staphylococcus aureus MW2

42.222

92.784

0.392
  comGC Staphylococcus aureus N315

42.222

92.784

0.392