Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RAM33_RS02850 Genome accession   NZ_CP132372
Coordinates   512142..512639 (+) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SA3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 507142..517639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM33_RS02810 (RAM33_02805) - 507186..508172 (+) 987 WP_000161311.1 ROK family glucokinase -
  RAM33_RS02815 (RAM33_02810) - 508172..508501 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM33_RS02820 (RAM33_02815) - 508498..509121 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RAM33_RS02825 (RAM33_02820) comGA 509173..510147 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM33_RS02830 (RAM33_02825) comGB 510119..511189 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM33_RS02835 (RAM33_02830) comGC 511203..511514 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM33_RS02840 (RAM33_02835) comGD 511492..511938 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM33_RS02845 (RAM33_02840) comGE 511925..512224 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  RAM33_RS02850 (RAM33_02845) comGF 512142..512639 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM33_RS02855 (RAM33_02850) - 512736..512882 (+) 147 WP_001792109.1 hypothetical protein -
  RAM33_RS02860 (RAM33_02855) - 512872..513396 (+) 525 WP_001015120.1 shikimate kinase -
  RAM33_RS02865 (RAM33_02860) gcvT 513555..514646 (+) 1092 WP_306176509.1 glycine cleavage system aminomethyltransferase GcvT -
  RAM33_RS02870 (RAM33_02865) gcvPA 514666..516012 (+) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RAM33_RS02875 (RAM33_02870) gcvPB 516005..517477 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=868703 RAM33_RS02850 WP_001788897.1 512142..512639(+) (comGF) [Staphylococcus aureus strain UNC_SA3]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=868703 RAM33_RS02850 WP_001788897.1 512142..512639(+) (comGF) [Staphylococcus aureus strain UNC_SA3]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964