Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RAM33_RS02825 Genome accession   NZ_CP132372
Coordinates   509173..510147 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain UNC_SA3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 504173..515147
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM33_RS02790 (RAM33_02785) rpmG 504633..504782 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  RAM33_RS02795 (RAM33_02790) - 504991..505530 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  RAM33_RS02800 (RAM33_02795) - 505542..507005 (+) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  RAM33_RS02805 (RAM33_02800) - 506986..507189 (+) 204 WP_000087561.1 YqgQ family protein -
  RAM33_RS02810 (RAM33_02805) - 507186..508172 (+) 987 WP_000161311.1 ROK family glucokinase -
  RAM33_RS02815 (RAM33_02810) - 508172..508501 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM33_RS02820 (RAM33_02815) - 508498..509121 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RAM33_RS02825 (RAM33_02820) comGA 509173..510147 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM33_RS02830 (RAM33_02825) comGB 510119..511189 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM33_RS02835 (RAM33_02830) comGC 511203..511514 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM33_RS02840 (RAM33_02835) comGD 511492..511938 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM33_RS02845 (RAM33_02840) comGE 511925..512224 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  RAM33_RS02850 (RAM33_02845) comGF 512142..512639 (+) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM33_RS02855 (RAM33_02850) - 512736..512882 (+) 147 WP_001792109.1 hypothetical protein -
  RAM33_RS02860 (RAM33_02855) - 512872..513396 (+) 525 WP_001015120.1 shikimate kinase -
  RAM33_RS02865 (RAM33_02860) gcvT 513555..514646 (+) 1092 WP_306176509.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=868698 RAM33_RS02825 WP_000697220.1 509173..510147(+) (comGA) [Staphylococcus aureus strain UNC_SA3]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=868698 RAM33_RS02825 WP_000697220.1 509173..510147(+) (comGA) [Staphylococcus aureus strain UNC_SA3]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1