Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   RAM30_RS05040 Genome accession   NZ_CP132371
Coordinates   1031001..1031975 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain UNC_SA38     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1026001..1036975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM30_RS05000 (RAM30_04995) gcvT 1026502..1027593 (-) 1092 Protein_956 glycine cleavage system aminomethyltransferase GcvT -
  RAM30_RS05005 (RAM30_05000) - 1027752..1028276 (-) 525 WP_001015120.1 shikimate kinase -
  RAM30_RS05010 (RAM30_05005) - 1028266..1028412 (-) 147 WP_001792109.1 hypothetical protein -
  RAM30_RS05015 (RAM30_05010) comGF 1028509..1029006 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM30_RS05020 (RAM30_05015) comGE 1028924..1029223 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  RAM30_RS05025 (RAM30_05020) comGD 1029210..1029656 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM30_RS05030 (RAM30_05025) comGC 1029634..1029945 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM30_RS05035 (RAM30_05030) comGB 1029959..1031029 (-) 1071 WP_306177965.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM30_RS05040 (RAM30_05035) comGA 1031001..1031975 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM30_RS05045 (RAM30_05040) - 1032027..1032650 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RAM30_RS05050 (RAM30_05045) - 1032647..1032976 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM30_RS05055 (RAM30_05050) - 1032976..1033962 (-) 987 WP_000161311.1 ROK family glucokinase -
  RAM30_RS05060 (RAM30_05055) - 1033959..1034162 (-) 204 WP_000087561.1 YqgQ family protein -
  RAM30_RS05065 (RAM30_05060) - 1034143..1035606 (-) 1464 WP_001017852.1 rhomboid family intramembrane serine protease -
  RAM30_RS05070 (RAM30_05065) - 1035618..1036157 (-) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  RAM30_RS05075 (RAM30_05070) rpmG 1036366..1036515 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=868661 RAM30_RS05040 WP_000697220.1 1031001..1031975(-) (comGA) [Staphylococcus aureus strain UNC_SA38]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=868661 RAM30_RS05040 WP_000697220.1 1031001..1031975(-) (comGA) [Staphylococcus aureus strain UNC_SA38]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1