Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   RAM30_RS05035 Genome accession   NZ_CP132371
Coordinates   1029959..1031029 (-) Length   356 a.a.
NCBI ID   WP_306177965.1    Uniprot ID   -
Organism   Staphylococcus aureus strain UNC_SA38     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024959..1036029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAM30_RS04995 (RAM30_04990) gcvPA 1025136..1026482 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RAM30_RS05000 (RAM30_04995) gcvT 1026502..1027593 (-) 1092 Protein_956 glycine cleavage system aminomethyltransferase GcvT -
  RAM30_RS05005 (RAM30_05000) - 1027752..1028276 (-) 525 WP_001015120.1 shikimate kinase -
  RAM30_RS05010 (RAM30_05005) - 1028266..1028412 (-) 147 WP_001792109.1 hypothetical protein -
  RAM30_RS05015 (RAM30_05010) comGF 1028509..1029006 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  RAM30_RS05020 (RAM30_05015) comGE 1028924..1029223 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  RAM30_RS05025 (RAM30_05020) comGD 1029210..1029656 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RAM30_RS05030 (RAM30_05025) comGC 1029634..1029945 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RAM30_RS05035 (RAM30_05030) comGB 1029959..1031029 (-) 1071 WP_306177965.1 competence type IV pilus assembly protein ComGB Machinery gene
  RAM30_RS05040 (RAM30_05035) comGA 1031001..1031975 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  RAM30_RS05045 (RAM30_05040) - 1032027..1032650 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RAM30_RS05050 (RAM30_05045) - 1032647..1032976 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RAM30_RS05055 (RAM30_05050) - 1032976..1033962 (-) 987 WP_000161311.1 ROK family glucokinase -
  RAM30_RS05060 (RAM30_05055) - 1033959..1034162 (-) 204 WP_000087561.1 YqgQ family protein -
  RAM30_RS05065 (RAM30_05060) - 1034143..1035606 (-) 1464 WP_001017852.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41413.83 Da        Isoelectric Point: 10.0606

>NTDB_id=868660 RAM30_RS05035 WP_306177965.1 1029959..1031029(-) (comGB) [Staphylococcus aureus strain UNC_SA38]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIIIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=868660 RAM30_RS05035 WP_306177965.1 1029959..1031029(-) (comGB) [Staphylococcus aureus strain UNC_SA38]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCATTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.719

100

0.997
  comGB Staphylococcus aureus N315

99.719

100

0.997