Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RAB71_RS03535 Genome accession   NZ_CP132343
Coordinates   806452..807591 (-) Length   379 a.a.
NCBI ID   WP_010340231.1    Uniprot ID   A0A2P5Z2H8
Organism   Xanthomonas sacchari strain CFBP4641     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801452..812591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RAB71_RS03515 (RAB71_03515) queD 801800..802159 (-) 360 WP_010343828.1 6-carboxytetrahydropterin synthase QueD -
  RAB71_RS03520 (RAB71_03520) - 802393..803700 (-) 1308 Protein_700 DEAD/DEAH box helicase -
  RAB71_RS03525 (RAB71_03525) - 804046..805035 (+) 990 WP_010343830.1 fumarylacetoacetate hydrolase family protein -
  RAB71_RS03530 (RAB71_03530) maiA 805125..805781 (+) 657 WP_010343831.1 maleylacetoacetate isomerase -
  RAB71_RS03535 (RAB71_03535) pilU 806452..807591 (-) 1140 WP_010340231.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RAB71_RS03540 (RAB71_03540) - 807691..808083 (+) 393 WP_010340230.1 DUF4398 domain-containing protein -
  RAB71_RS03545 (RAB71_03545) - 808101..808973 (+) 873 WP_010340229.1 OmpA family protein -
  RAB71_RS03550 (RAB71_03550) - 809307..809525 (+) 219 WP_010340228.1 YdcH family protein -
  RAB71_RS03555 (RAB71_03555) - 809716..811086 (+) 1371 WP_010340227.1 pyridoxal-phosphate dependent enzyme -
  RAB71_RS03560 (RAB71_03560) - 811169..812353 (+) 1185 WP_010340226.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42110.40 Da        Isoelectric Point: 6.2384

>NTDB_id=868289 RAB71_RS03535 WP_010340231.1 806452..807591(-) (pilU) [Xanthomonas sacchari strain CFBP4641]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDYRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLSLNLRAVISQRLVKDNTGRRRPASEVLLNTPMIRDLLRRGQVHEIKQAMEESLEDGMETFDQCL
FRMVKQGQIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYDSGPSAAPRITHGFV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=868289 RAB71_RS03535 WP_010340231.1 806452..807591(-) (pilU) [Xanthomonas sacchari strain CFBP4641]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACAGGAGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGGGAGCTGGAATTGAACATGGCCATCGCCCTGCAGGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAGCTGCACCTGCCGCAGGTGCTCAAGGACGTGATCATGACCCCGCGCGGGCTGGTCCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACTACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCCATCGTCAACCAGCGCGAGGTGGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCAATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAGACCATGGAGGCGG
CCATTGCCTTCGCCGAGACCGGGCACCTGTGTCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGTTGATGAACCTGTCGCTGAACCTGCGCGCAGTGATCTCGCAGCG
TCTGGTCAAGGACAACACCGGCCGCCGCCGGCCGGCCTCGGAAGTGCTGCTGAACACGCCGATGATCCGCGACCTGCTGC
GGCGCGGGCAGGTCCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGACGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGCAGGGCCAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCCCTGAA
GTTCCGCCTGTCCGAAGGCGGCAGCGGCGAACACGACCCCTACGCCGACTACGACAGCGGCCCCAGCGCCGCGCCGCGCA
TCACCCACGGCTTCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2P5Z2H8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

94.723

0.493
  pilU Vibrio cholerae strain A1552

49.722

94.987

0.472
  pilU Acinetobacter baylyi ADP1

50

93.404

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.915

99.736

0.438
  pilT Pseudomonas aeruginosa PAK

43.491

89.182

0.388
  pilT Acinetobacter baumannii D1279779

42.604

89.182

0.38
  pilT Acinetobacter baumannii strain A118

42.604

89.182

0.38
  pilT Acinetobacter nosocomialis M2

42.604

89.182

0.38
  pilT Acinetobacter baylyi ADP1

41.471

89.71

0.372
  pilT Pseudomonas stutzeri DSM 10701

41.716

89.182

0.372
  pilT Legionella pneumophila strain ERS1305867

41.716

89.182

0.372
  pilT Legionella pneumophila strain Lp02

41.716

89.182

0.372
  pilT Neisseria meningitidis 8013

40.346

91.557

0.369
  pilT Neisseria gonorrhoeae MS11

40.346

91.557

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364