Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   Q7W79_RS08540 Genome accession   NZ_CP131931
Coordinates   154008..154601 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain RP0132     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 149008..159601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7W79_RS08525 (Q7W79_08525) - 149725..152295 (-) 2571 WP_005480476.1 penicillin-binding protein 1A -
  Q7W79_RS08530 (Q7W79_08530) pilM 152438..153453 (+) 1016 Protein_127 type IV pilus biogenesis protein PilM -
  Q7W79_RS08535 (Q7W79_08535) pilN 153437..154015 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  Q7W79_RS08540 (Q7W79_08540) pilO 154008..154601 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  Q7W79_RS08545 (Q7W79_08545) pilP 154591..155106 (+) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  Q7W79_RS08550 (Q7W79_08550) pilQ 155138..156886 (+) 1749 WP_025623689.1 type IV pilus secretin PilQ Machinery gene
  Q7W79_RS08555 (Q7W79_08555) aroK 157075..157593 (+) 519 WP_005381319.1 shikimate kinase AroK -
  Q7W79_RS08560 (Q7W79_08560) aroB 157625..158725 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=866331 Q7W79_RS08540 WP_005480482.1 154008..154601(+) (pilO) [Vibrio parahaemolyticus strain RP0132]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=866331 Q7W79_RS08540 WP_005480482.1 154008..154601(+) (pilO) [Vibrio parahaemolyticus strain RP0132]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCCAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726