Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   WAC27_RS08275 Genome accession   NZ_CP148034
Coordinates   1703213..1705294 (+) Length   693 a.a.
NCBI ID   WP_000678417.1    Uniprot ID   -
Organism   Escherichia coli strain NH-21     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1698213..1710294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAC27_RS08255 (WAC27_08255) gmk 1699431..1700054 (+) 624 WP_001295237.1 guanylate kinase -
  WAC27_RS08260 (WAC27_08260) rpoZ 1700109..1700384 (+) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  WAC27_RS08265 (WAC27_08265) spoT 1700403..1702511 (+) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  WAC27_RS08270 (WAC27_08270) trmH 1702518..1703207 (+) 690 WP_001070177.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  WAC27_RS08275 (WAC27_08275) recG 1703213..1705294 (+) 2082 WP_000678417.1 ATP-dependent DNA helicase RecG Machinery gene
  WAC27_RS08280 (WAC27_08280) gltS 1705463..1706668 (-) 1206 WP_000468836.1 sodium/glutamate symporter -
  WAC27_RS08285 (WAC27_08285) xanP 1706948..1708339 (+) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  WAC27_RS08290 (WAC27_08290) yicH 1708460..1710169 (+) 1710 WP_001295239.1 AsmA family protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76416.41 Da        Isoelectric Point: 7.3384

>NTDB_id=863656 WAC27_RS08275 WP_000678417.1 1703213..1705294(+) (recG) [Escherichia coli strain NH-21]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIERDLALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVLFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=863656 WAC27_RS08275 WP_000678417.1 1703213..1705294(+) (recG) [Escherichia coli strain NH-21]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGCGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATCCTCACCATGCGCTTTTTCAATTTCAGCGCGGCAATGAAAAATAGCCT
GGCGGCGGGCCGCCGTGTACTGGCTTATGGAGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAAGGCGATCTCAGCACGCCAGAGTTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTGCCAGAAGCGTTGCGCACTTTGCATCGCCCGCCACCGACTTTGCAGCTTAGCGATCTGG
AAACCGGACAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCATAACCTCAGCATGTTAGCCTTACGC
GCCGGAGCGCAACGTTTCCATGCCCAGCCGCTGAGCGCCAATGACACGCTGAAAAATAAACTTCTTGCCGCCTTACCGTT
CAAGCCAACGGGCGCTCAGGCTCGCGTGGTGGCGGAGATCGAGCGCGATTTGGCGCTGGATGTGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCTGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAACAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTTGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCGCGGCTGGCACAGCAGGAAGCTATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGTACACACGCCATCTTCCAGGAACAGGTGCTGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCGTTATGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCTCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGAACGCTGGCAATGACTGCGTATGCTGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATTATTGACCGCGTGCGCCACGCCTGCATA
ACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTTGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTGCGCGGGCGCGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGC
GCGACAGTAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCACGCCA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

RecG wedge domain (RecG_wedge)

(15-171)

DEAD/DEAH box helicase (DEAD)

(292-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.381

96.97

0.508
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.058

94.949

0.371