Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   WFE12_RS08700 Genome accession   NZ_CP147877
Coordinates   1661225..1663273 (+) Length   682 a.a.
NCBI ID   WP_003244692.1    Uniprot ID   O34942
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1656225..1668273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WFE12_RS08670 spoVM 1656861..1656941 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  WFE12_RS08675 rpmB 1657014..1657202 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  WFE12_RS08680 yloU 1657479..1657841 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  WFE12_RS08685 yloV 1657857..1659518 (+) 1662 WP_003245033.1 DAK2 domain-containing protein -
  WFE12_RS08690 sdaAB 1659657..1660319 (+) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  WFE12_RS08695 sdaAA 1660345..1661247 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  WFE12_RS08700 recG 1661225..1663273 (+) 2049 WP_003244692.1 ATP-dependent DNA helicase RecG Machinery gene
  WFE12_RS08705 fapR 1663382..1663948 (+) 567 WP_003232044.1 transcription factor FapR -
  WFE12_RS08710 plsX 1663962..1664963 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  WFE12_RS08715 fabD 1664982..1665935 (+) 954 WP_003245314.1 ACP S-malonyltransferase -
  WFE12_RS08720 fabG 1665928..1666668 (+) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  WFE12_RS08725 acpP 1666752..1666985 (+) 234 WP_003154310.1 acyl carrier protein -
  WFE12_RS08730 rncS 1667125..1667874 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78140.72 Da        Isoelectric Point: 7.4260

>NTDB_id=863285 WFE12_RS08700 WP_003244692.1 1661225..1663273(+) (recG) [Bacillus subtilis subsp. subtilis str. 168]
MKQHQQTSIANIKGIGPETEKTLNELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=863285 WFE12_RS08700 WP_003244692.1 1661225..1663273(+) (recG) [Bacillus subtilis subsp. subtilis str. 168]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTGAATGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAGCGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGTCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGAAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCATGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGTTGAGTCTCGTTATTACTGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACATTAGCGATCACAGTATTCGGTGAAAT
GGATGTATCTGTCATTGATGAGATGCCGGCTGGACGAAAGCGAATTGAAACCTATTGGGTAAAACATGACATGCTTGATC
GTATTTTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAT
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGATCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CCACTGTTGTGGAGGTTGGCGTAAATGTTCCGAATGCAACAATTATGGTGATTTATGACGCCGACCGTTTCGGACTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGGAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O34942

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

100

100

1
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38