Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   I0P84_RS07635 Genome accession   NZ_AP024524
Coordinates   1563147..1564343 (+) Length   398 a.a.
NCBI ID   WP_214002257.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain RYU24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1558147..1569343
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0P84_RS07620 (RYU24_15020) - 1559178..1559708 (+) 531 WP_004785566.1 hypothetical protein -
  I0P84_RS07625 (RYU24_15030) - 1560261..1561310 (+) 1050 WP_214002256.1 3-deoxy-7-phosphoheptulonate synthase -
  I0P84_RS07630 (RYU24_15040) - 1561565..1562743 (+) 1179 Protein_1490 PilT/PilU family type 4a pilus ATPase -
  I0P84_RS07635 (RYU24_15050) pilU 1563147..1564343 (+) 1197 WP_214002257.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  I0P84_RS07640 (RYU24_15060) - 1564419..1565594 (-) 1176 WP_214002258.1 MFS transporter -
  I0P84_RS07645 (RYU24_15070) gcvH 1565847..1566221 (-) 375 WP_104426083.1 glycine cleavage system protein GcvH -
  I0P84_RS07650 (RYU24_15080) - 1566304..1567332 (-) 1029 WP_214002259.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 44972.53 Da        Isoelectric Point: 6.5072

>NTDB_id=85808 I0P84_RS07635 WP_214002257.1 1563147..1564343(+) (pilU) [Acinetobacter variabilis strain RYU24]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQILTAEKTKLFAYSLMNDKQRQEFEAELEC
NFAITVPGVSRFRVNVFVQQLQVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYIHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLISDLILKGEFHELKAIMGKS
RELGMQTFDQALFDLYNEGAISYEEALRNADSTNELRLQIKLKSNRQDNPAAGEATQFTMMQEPKAEEKEQENTSASE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=85808 I0P84_RS07635 WP_214002257.1 1563147..1564343(+) (pilU) [Acinetobacter variabilis strain RYU24]
ATGTTCACGGCAGAACTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACCAAAGTAGTGGAATATGGGGGCTC
AGACTTATTTATTACCGCAGATTTTCCACCGAGTATTAAACATCAGGGCTTAATGAAGCCCTTTGGACAACAAATTCTTA
CTGCTGAAAAAACCAAACTGTTTGCTTATAGCTTGATGAATGATAAACAGCGTCAAGAGTTTGAAGCTGAACTAGAATGT
AACTTCGCGATTACTGTACCGGGTGTATCGCGCTTCCGGGTAAATGTTTTTGTACAACAGCTTCAGGTCGGGATGGTAAT
CCGGACCATTACGGCAGAAATTCCAAATTTCCAGAAACTGAAATTACCAGAATCATTGAAACACGTGATTATGGAAAAAC
GCGGTCTGGTACTGGTTGTAGGAGGAACGGGTTCAGGTAAATCGACCTCTCTGGCAGCGATGATTGACTACCGCAATGAA
AATTCGGCGGGGCATATCATTACCGTGGAAGATCCGGTGGAATATATACATAAACACAAGAAATCCATGATTACTCACCG
TGAAGTTGGAGTCGATTGCCATTCCTGGCACAATGCGCTGAAAAATACCTTGCGTCAGGCCCCCGATGTAATTCTGATCG
GTGAGATTCGGGATACCGAAACCATGGAACACGCAATTGCCTTTGCTGAAACCGGACATTTATGTCTGGGTACACTGCAT
TCCAATAATGCTAACCAGACTTTGGACCGGATTATTAACTTCTTCCCGGAAGAACGCCGTAATCAGTTATTAATGGATTT
ATCTTCGAATATGAAGGCAATTATTTCCCAGCGTCTGATTCGTACTCAGGATGGGAAAGGCCGGCGTGCTGCAATTGAAA
TCATGTTAAATACACCTTTGATTTCTGATCTGATTCTAAAAGGTGAGTTTCATGAGCTGAAAGCCATTATGGGCAAATCT
CGTGAACTGGGCATGCAGACTTTCGATCAGGCCTTATTTGACCTGTATAACGAGGGTGCGATTTCTTATGAGGAAGCATT
GCGTAATGCTGATTCGACTAATGAATTACGTTTACAGATCAAGCTGAAAAGCAACCGCCAGGATAATCCTGCTGCTGGAG
AAGCAACACAATTCACGATGATGCAAGAACCGAAAGCTGAAGAAAAAGAACAAGAAAATACTTCAGCATCTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.207

87.437

0.535
  pilU Acinetobacter baylyi ADP1

56.196

87.186

0.49
  pilU Vibrio cholerae strain A1552

51.507

91.709

0.472

Multiple sequence alignment