Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   I0P84_RS01945 Genome accession   NZ_AP024524
Coordinates   423149..423601 (+) Length   150 a.a.
NCBI ID   WP_175966207.1    Uniprot ID   -
Organism   Acinetobacter variabilis strain RYU24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 400333..424062 423149..423601 within 0


Gene organization within MGE regions


Location: 400333..424062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I0P84_RS01825 (RYU24_03520) - 400333..400638 (+) 306 WP_000122230.1 IS630 transposase-related protein -
  I0P84_RS15545 - 400722..401080 (+) 359 Protein_353 transposase -
  I0P84_RS01840 (RYU24_03530) - 401182..402303 (+) 1122 Protein_354 hypothetical protein -
  I0P84_RS01845 - 402300..404357 (+) 2058 Protein_355 hypothetical protein -
  I0P84_RS01850 (RYU24_03560) - 404361..404720 (+) 360 WP_180033744.1 hypothetical protein -
  I0P84_RS01855 (RYU24_03570) - 404742..405236 (-) 495 WP_228301766.1 pilin -
  I0P84_RS01860 (RYU24_03580) - 405784..405954 (+) 171 WP_214001844.1 hypothetical protein -
  I0P84_RS01865 (RYU24_03590) - 406198..406653 (+) 456 WP_214001845.1 pilin -
  I0P84_RS01870 (RYU24_03600) tfpZ 406809..407543 (+) 735 WP_214001846.1 TfpX/TfpZ family type IV pilin accessory protein -
  I0P84_RS01875 (RYU24_03610) - 407629..409263 (+) 1635 WP_214001847.1 PglL family O-oligosaccharyltransferase -
  I0P84_RS01880 (RYU24_03620) bfr 409318..409782 (-) 465 WP_214001848.1 heteropolymeric bacterioferritin subunit Bfr -
  I0P84_RS01885 (RYU24_03630) - 410009..410203 (-) 195 WP_004787097.1 bacterioferritin-associated ferredoxin -
  I0P84_RS01890 (RYU24_03650) - 410425..410808 (-) 384 WP_104425645.1 RidA family protein -
  I0P84_RS01895 (RYU24_03660) - 410941..412002 (+) 1062 WP_214001849.1 RNA-guided endonuclease InsQ/TnpB family protein -
  I0P84_RS01900 (RYU24_03670) - 412079..414187 (-) 2109 WP_214001850.1 RelA/SpoT family protein -
  I0P84_RS01905 (RYU24_03680) rpoZ 414405..414686 (-) 282 WP_004650579.1 DNA-directed RNA polymerase subunit omega -
  I0P84_RS01910 (RYU24_03690) gmk 414764..415387 (-) 624 WP_004787090.1 guanylate kinase -
  I0P84_RS01915 (RYU24_03700) ispH 415523..416473 (+) 951 WP_075167883.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  I0P84_RS01920 (RYU24_03710) - 416637..417083 (+) 447 WP_214001851.1 pilus assembly FimT family protein -
  I0P84_RS01925 (RYU24_03720) pilV 417083..417631 (+) 549 WP_175966214.1 type IV pilus modification protein PilV -
  I0P84_RS01930 (RYU24_03730) - 417633..418670 (+) 1038 WP_214001852.1 PilW family protein -
  I0P84_RS01935 (RYU24_03740) - 418667..419434 (+) 768 WP_214001853.1 pilus assembly PilX family protein -
  I0P84_RS01940 (RYU24_03750) - 419450..423139 (+) 3690 WP_214001854.1 pilus assembly protein -
  I0P84_RS01945 (RYU24_03760) comE 423149..423601 (+) 453 WP_175966207.1 type IV pilin protein Machinery gene
  I0P84_RS01950 (RYU24_03770) - 423595..424062 (+) 468 WP_175966205.1 type IV pilin protein -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 16355.44 Da        Isoelectric Point: 9.0903

>NTDB_id=85798 I0P84_RS01945 WP_175966207.1 423149..423601(+) (comE) [Acinetobacter variabilis strain RYU24]
MNNALSIQKGFTLIELMVVVVIVAIIAAIAIPSYQEYARRANAAQAQQEVQRLASELERWKSRNFNYLGFNLTPNAVQNY
TFVVRDGAAGNPALTATAASGTSWVIRAVHNDGRNFSFLMSSNGIRCKNKTSSNVTFTDCGTDTTGSEAW

Nucleotide


Download         Length: 453 bp        

>NTDB_id=85798 I0P84_RS01945 WP_175966207.1 423149..423601(+) (comE) [Acinetobacter variabilis strain RYU24]
ATGAATAATGCATTATCTATTCAGAAAGGTTTTACCTTAATTGAGCTGATGGTTGTGGTGGTGATTGTTGCAATTATTGC
AGCAATCGCTATTCCAAGCTATCAGGAATATGCACGTAGAGCTAACGCAGCACAAGCACAGCAAGAGGTGCAGCGCTTAG
CAAGTGAACTTGAACGTTGGAAATCACGTAATTTTAATTATTTAGGCTTTAATCTTACTCCCAATGCTGTTCAAAACTAC
ACTTTTGTGGTTCGAGATGGTGCTGCGGGAAATCCAGCTCTTACAGCAACAGCAGCAAGCGGGACGAGTTGGGTGATACG
TGCTGTGCACAATGATGGTCGAAACTTTAGCTTCTTAATGAGTAGTAATGGAATTCGTTGTAAAAATAAAACCAGCAGCA
ATGTGACATTCACAGATTGTGGGACAGATACAACAGGGAGTGAGGCATGGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(32-112)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-30)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Acinetobacter baylyi ADP1

48.214

100

0.54
  pilY2 Acinetobacter baumannii D1279779

48.052

100

0.493

Multiple sequence alignment