Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   STYK_RS10075 Genome accession   NZ_AP024523
Coordinates   2022907..2024205 (-) Length   432 a.a.
NCBI ID   WP_261804994.1    Uniprot ID   -
Organism   Streptococcus toyakuensis strain TP1632     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2017907..2029205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  STYK_RS10055 (STYK_19320) rplI 2019062..2019514 (-) 453 WP_004255324.1 50S ribosomal protein L9 -
  STYK_RS10060 (STYK_19330) - 2019511..2021484 (-) 1974 WP_261804992.1 DHH family phosphoesterase -
  STYK_RS10065 (STYK_19340) hpf 2021620..2022168 (-) 549 WP_000599100.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  STYK_RS10070 (STYK_19350) comFC/cflB 2022248..2022910 (-) 663 WP_261804993.1 ComF family protein Machinery gene
  STYK_RS10075 (STYK_19360) comFA/cflA 2022907..2024205 (-) 1299 WP_261804994.1 DEAD/DEAH box helicase Machinery gene
  STYK_RS10080 (STYK_19370) - 2024262..2024897 (+) 636 WP_261804995.1 YigZ family protein -
  STYK_RS10290 - 2024991..2025086 (+) 96 Protein_1941 PH domain-containing protein -
  STYK_RS10085 (STYK_19380) cysK 2025183..2026109 (+) 927 WP_261804996.1 cysteine synthase A -
  STYK_RS10090 (STYK_19390) tsf 2026232..2027272 (-) 1041 WP_084887694.1 translation elongation factor Ts -
  STYK_RS10095 (STYK_19400) rpsB 2027351..2028130 (-) 780 WP_007556349.1 30S ribosomal protein S2 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49563.59 Da        Isoelectric Point: 8.8103

>NTDB_id=85778 STYK_RS10075 WP_261804994.1 2022907..2024205(-) (comFA/cflA) [Streptococcus toyakuensis strain TP1632]
MKVNPNYLGRLFTENELTKEERQLAEKLPAMRKEKGKLFCQRCDSAILDEWYLPIGAYYCRECLLMKRVRSDQVLYYFPQ
EDFAKQDILKWRGQLTPFQDKVSQGLLQAVDKQEPSLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQKDFACEIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
DELDRKVRIGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKSRLSPKLKSYIEKQRKTGYPLLIFASEIKKGEQLKEI
IQEQFPNEKIGFVSSITENRLEQVQAFRDRELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=85778 STYK_RS10075 WP_261804994.1 2022907..2024205(-) (comFA/cflA) [Streptococcus toyakuensis strain TP1632]
ATGAAAGTAAATCCAAATTATCTCGGTCGGTTGTTTACGGAGAATGAATTAACTAAAGAGGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTGATAGTGCTATTTTAGATGAATGGTATTTGC
CCATCGGTGCTTACTATTGTAGGGAGTGTTTGCTAATGAAGCGAGTCAGGAGTGATCAAGTTTTATACTATTTTCCGCAG
GAGGATTTTGCGAAGCAAGATATTCTTAAATGGCGTGGTCAATTAACTCCTTTTCAAGATAAGGTATCACAGGGACTGCT
TCAAGCGGTAGACAAGCAAGAGCCAAGCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TAGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCCAGTCCTCGCATAGACGTTTGTTTGGAGCTGTATAAGCGC
CTGCAAAAGGATTTTGCTTGTGAGATAGCCCTCCTACATGGAGAATCAGAGCCGTATTTTCGAACACCACTAGTTGTTGC
GACGACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTACTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCTATGCTTTACCATGCTGTCAAGAATAGTGTAAAGGAGAATGGCTTGAGAATCTTTTTAACAGCAACTTCTACC
GATGAGTTAGATAGGAAGGTTCGCATCGGAGAATTAAAACGATTGAGTTTGCCAAGACGATTTCATGGAAATCCTTTGAT
TATTCCTAAACCTGTCTGGTTATCCGATTTTAATCACTGTTTAGAGAAAAGTCGGTTGTCACCAAAGCTAAAGTCTTATA
TTGAGAAGCAGAGAAAGACAGGTTATCCGTTACTCATTTTTGCTTCAGAGATTAAGAAAGGGGAACAGTTAAAAGAAATC
ATACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCTTT
TCGAGATAGAGAACTGACAATACTTATCAGTACGACGATATTGGAGCGTGGAGTTACCTTCCCTTGTGTTGACGTTTTCG
TAGTAGAGGCCAATCATCGTCTGTTTACCAAGTCTAGTTTAATACAGATTGGAGGACGTGTCGGTCGGAGTATGGACAGA
CCGACAGGAGATTTGCTTTTCTTCCATGACGGGTTAAATGCTTCAATCAAAAAGGCAATTAAGGAAATTCAGCAGATGAA
TAAGGAGGCGGGGCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(292-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

97.917

100

0.979
  comFA/cflA Streptococcus mitis SK321

95.37

100

0.954
  comFA/cflA Streptococcus pneumoniae Rx1

92.593

100

0.926
  comFA/cflA Streptococcus pneumoniae D39

92.593

100

0.926
  comFA/cflA Streptococcus pneumoniae R6

92.593

100

0.926
  comFA/cflA Streptococcus pneumoniae TIGR4

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

50.117

99.306

0.498
  comFA Latilactobacillus sakei subsp. sakei 23K

38.391

100

0.387

Multiple sequence alignment