Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   Q0G00_RS12285 Genome accession   NZ_CP129978
Coordinates   2489652..2491037 (+) Length   461 a.a.
NCBI ID   WP_029091568.1    Uniprot ID   A0A1D2KGY7
Organism   Brochothrix thermosphacta strain DH-B18     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2484652..2496037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q0G00_RS12275 (Q0G00_12275) - 2486455..2486913 (+) 459 WP_029091570.1 CtsR family transcriptional regulator -
  Q0G00_RS12280 (Q0G00_12280) - 2486944..2489445 (+) 2502 WP_069127339.1 ATP-dependent Clp protease ATP-binding subunit -
  Q0G00_RS12285 (Q0G00_12285) radA 2489652..2491037 (+) 1386 WP_029091568.1 DNA repair protein RadA Machinery gene
  Q0G00_RS12290 (Q0G00_12290) - 2491053..2492135 (+) 1083 WP_029091567.1 PIN/TRAM domain-containing protein -
  Q0G00_RS12295 (Q0G00_12295) ispD 2492149..2492847 (+) 699 WP_069129620.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  Q0G00_RS12300 (Q0G00_12300) ispF 2492850..2493326 (+) 477 WP_029091565.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  Q0G00_RS12305 (Q0G00_12305) gltX 2493362..2494830 (+) 1469 Protein_2372 glutamate--tRNA ligase -
  Q0G00_RS12310 (Q0G00_12310) epsC 2495147..2495785 (+) 639 WP_029091563.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50638.30 Da        Isoelectric Point: 6.4051

>NTDB_id=856816 Q0G00_RS12285 WP_029091568.1 2489652..2491037(+) (radA) [Brochothrix thermosphacta strain DH-B18]
MAKKKASTKFVCNACGYESPKWMGRCPNCSEWNQMVEFVEAKVQKGRTSFNHTEEMESQQALPLSKITSETEQRILTDMP
ELNRVLGGGLVKGSLVLVGGDPGIGKSTLLLQVSAQLTTAKRKGLYVSGEESIKQTKLRAERLNVDGDLLYVYAQTDLEV
ISKTIQTLQPEFVVIDSIQTIYHPDVTSAAGSVTQVRECTSELMRLAKTLNIAIFIVGHVTKEGTIAGPRMLEHMVDTVL
YFEGERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVDNASEVFLEERLEGAAGSAVVTSMEGTRPVLVEIQALVSPT
VYGNARRMATGMDYNRVSLIMAVLEKREGLMLQNQDAYLKAAGGVKLDEPAIDLAIAISIASSYYDTPTAAMDCFIGEVG
LTGELRRVARIDQRVQEAAKLGFKRIIIPANNIGGWTFPAEIEVVGVSTLKDAIHKAFPNR

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=856816 Q0G00_RS12285 WP_029091568.1 2489652..2491037(+) (radA) [Brochothrix thermosphacta strain DH-B18]
ATGGCAAAGAAAAAAGCGAGTACTAAATTTGTCTGCAACGCCTGCGGATATGAGTCTCCAAAATGGATGGGGCGTTGCCC
TAACTGTAGCGAATGGAACCAAATGGTCGAATTTGTTGAGGCAAAAGTCCAAAAAGGACGAACTAGTTTTAATCATACAG
AAGAAATGGAAAGTCAGCAGGCATTGCCTCTTTCTAAAATAACAAGTGAAACTGAACAACGTATCTTAACAGATATGCCA
GAATTGAATCGTGTCCTCGGCGGGGGATTAGTTAAAGGTTCATTGGTGTTGGTCGGTGGTGACCCAGGGATTGGTAAATC
AACACTATTGTTGCAAGTATCAGCACAACTCACAACAGCAAAACGCAAAGGCCTTTATGTTTCGGGAGAAGAATCAATCA
AGCAAACAAAACTACGGGCTGAACGTTTAAATGTGGATGGGGATTTATTATATGTTTATGCGCAAACCGACTTAGAAGTC
ATCTCTAAAACAATTCAGACTTTACAGCCGGAATTTGTCGTGATTGATTCGATCCAAACCATCTATCACCCTGATGTGAC
AAGTGCAGCAGGGAGCGTCACACAAGTAAGAGAGTGTACGTCAGAATTGATGCGTCTTGCAAAGACGTTAAACATTGCCA
TCTTCATTGTCGGTCATGTGACAAAAGAGGGTACAATTGCAGGTCCACGTATGTTAGAACATATGGTTGACACTGTTTTA
TACTTTGAAGGTGAACGCCACCATGCCTACCGGATTTTACGCGCCGTAAAAAATAGGTTTGGATCTACCAATGAAATGGG
TATCTTTGAAATGCGCGATGTCGGTTTGGTAGAGGTCGACAACGCCTCAGAAGTATTTTTAGAAGAGCGTTTAGAAGGAG
CTGCTGGTTCTGCAGTTGTTACCTCAATGGAGGGGACACGTCCTGTTCTGGTAGAGATTCAAGCATTGGTGTCACCGACT
GTTTATGGAAATGCACGTCGAATGGCAACAGGAATGGATTATAATCGTGTTTCTCTAATTATGGCGGTACTTGAGAAACG
TGAAGGTTTAATGCTTCAAAATCAAGATGCCTATCTTAAAGCAGCAGGTGGTGTGAAGTTAGACGAGCCTGCAATTGATT
TAGCAATAGCAATAAGTATTGCATCGAGTTATTATGATACACCAACAGCAGCAATGGATTGCTTTATTGGTGAAGTCGGT
TTAACGGGAGAGTTACGTCGTGTTGCTCGTATTGATCAACGCGTACAAGAAGCTGCAAAACTAGGTTTCAAACGTATTAT
TATACCAGCGAACAATATCGGTGGTTGGACATTCCCTGCTGAGATTGAAGTAGTAGGTGTATCAACATTAAAAGATGCGA
TTCATAAAGCATTTCCAAATAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

AAA domain (AAA_25)

(66-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D2KGY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.756

99.566

0.675
  radA Streptococcus pneumoniae Rx1

64.13

99.783

0.64
  radA Streptococcus pneumoniae D39

64.13

99.783

0.64
  radA Streptococcus pneumoniae R6

64.13

99.783

0.64
  radA Streptococcus pneumoniae TIGR4

64.13

99.783

0.64
  radA Streptococcus mitis SK321

64.13

99.783

0.64
  radA Streptococcus mitis NCTC 12261

64.13

99.783

0.64