Detailed information NTG0856117

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: QZH48_RS05500
• Genome accession: NZ_CP129879
• Coordinates: 1111174..1112496 (-)
• Length: 440 a.a.
• NCBI ID: WP_146977985.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain CAB701
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1106174..1117496

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QZH48_RS05470 (QZH48_05470)	-	1107316..1108284 (+)	969	WP_003130649.1	PhoH family protein	-
QZH48_RS05475 (QZH48_05475)	-	1108342..1108821 (+)	480	WP_098393891.1	NUDIX hydrolase	-
QZH48_RS05480 (QZH48_05480)	ybeY	1108973..1109461 (+)	489	WP_003130652.1	rRNA maturation RNase YbeY	-
QZH48_RS05485 (QZH48_05485)	-	1109445..1109900 (+)	456	WP_003130653.1	diacylglycerol kinase family protein	-
QZH48_RS05490 (QZH48_05490)	-	1109914..1110492 (+)	579	WP_098393890.1	nucleotidyltransferase family protein	-
QZH48_RS05495 (QZH48_05495)	-	1110527..1111177 (-)	651	WP_058205632.1	ComF family protein	-
QZH48_RS05500 (QZH48_05500)	comFA	1111174..1112496 (-)	1323	WP_146977985.1	DEAD/DEAH box helicase	Machinery gene
QZH48_RS05505 (QZH48_05505)	-	1112553..1113182 (+)	630	WP_098393888.1	YigZ family protein	-
QZH48_RS05510 (QZH48_05510)	-	1113316..1113813 (+)	498	WP_038601222.1	VanZ family protein	-
QZH48_RS05515 (QZH48_05515)	-	1113861..1114880 (+)	1020	WP_038601219.1	DUF475 domain-containing protein	-
QZH48_RS05520 (QZH48_05520)	-	1114995..1115741 (+)	747	WP_038601216.1	bifunctional metallophosphatase/5'-nucleotidase	-
QZH48_RS05525 (QZH48_05525)	glyQ	1116026..1116979 (+)	954	WP_146977987.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50243.84 Da
• Isoelectric Point: 10.1468

>NTDB_id=856117 QZH48_RS05500 WP_146977985.1 1111174..1112496(-) (comFA) [Lactococcus lactis subsp. lactis strain CAB701]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLIETTVKSILGMTSITSNKIKCNRCGTFHIKNAVKLPIGAFYC
PSCIQLGRVRSDEFLYFLPQKIFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILEK
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLVEQRNSGFPLLIFAAEIES
GQEFAKQLQLKFPKEKIAFVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=856117 QZH48_RS05500 WP_146977985.1 1111174..1112496(-) (comFA) [Lactococcus lactis subsp. lactis strain CAB701]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGATAGAAACAACGGTAAAATCAATTTTGGGGATGACTTCAATTACTT
CCAATAAAATAAAATGCAACCGCTGTGGGACTTTTCATATAAAAAATGCTGTCAAACTTCCAATTGGTGCATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTTCCACAAAAGATTTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGGGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAAA
GGTGGAGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GCTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAGGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGGAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTGCCGCGTCGTTTTCACAATTTTCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAGTTGAGCAAAGAAATAGTGGCTTTCCACTTCTAATTTTTGCTGCTGAAATTGAATCT
GGACAAGAATTTGCAAAACAACTACAACTAAAATTTCCTAAAGAAAAAATTGCTTTCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCTGCACTGATACAAATGGCTGGACGT
GTAGGTCGTAGTCCTGAGCGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-232)

• Helicase conserved C-terminal domain (Helicase_C) (301-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.5	90.909	0.677
comFA/cflA	Streptococcus pneumoniae Rx1	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae D39	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae R6	52.513	90.455	0.475
comFA/cflA	Streptococcus pneumoniae TIGR4	52.513	90.455	0.475
comFA/cflA	Streptococcus mitis SK321	52.01	90.455	0.47
comFA/cflA	Streptococcus mitis NCTC 12261	51.256	90.455	0.464
comFA	Bacillus subtilis subsp. subtilis str. 168	37.029	100	0.38