Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   QYT32_RS01815 Genome accession   NZ_CP129553
Coordinates   339186..339683 (+) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 704D     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 334186..344683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYT32_RS01775 (QYT32_01770) - 334230..335216 (+) 987 WP_000161314.1 ROK family glucokinase -
  QYT32_RS01780 (QYT32_01775) - 335216..335545 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  QYT32_RS01785 (QYT32_01780) - 335542..336165 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  QYT32_RS01790 (QYT32_01785) comGA 336217..337191 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  QYT32_RS01795 (QYT32_01790) comGB 337163..338254 (+) 1092 WP_123089976.1 competence type IV pilus assembly protein ComGB Machinery gene
  QYT32_RS01800 (QYT32_01795) comGC 338247..338558 (+) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  QYT32_RS01805 (QYT32_01800) comGD 338536..338982 (+) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  QYT32_RS01810 (QYT32_01805) comGE 338969..339268 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  QYT32_RS01815 (QYT32_01810) comGF 339186..339683 (+) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  QYT32_RS01820 (QYT32_01815) - 339780..339926 (+) 147 WP_001789879.1 hypothetical protein -
  QYT32_RS01825 (QYT32_01820) - 339916..340440 (+) 525 WP_001015125.1 shikimate kinase -
  QYT32_RS01830 (QYT32_01825) gcvT 340599..341690 (+) 1092 WP_123089974.1 glycine cleavage system aminomethyltransferase GcvT -
  QYT32_RS01835 (QYT32_01830) gcvPA 341710..343056 (+) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  QYT32_RS01840 (QYT32_01835) gcvPB 343049..344521 (+) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=854217 QYT32_RS01815 WP_001794328.1 339186..339683(+) (comGF) [Staphylococcus aureus strain 704D]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=854217 QYT32_RS01815 WP_001794328.1 339186..339683(+) (comGF) [Staphylococcus aureus strain 704D]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976