Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QY488_RS23485 Genome accession   NZ_CP129517
Coordinates   5072680..5073552 (+) Length   290 a.a.
NCBI ID   WP_003112839.1    Uniprot ID   P22610
Organism   Pseudomonas aeruginosa PAO1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5067680..5078552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QY488_RS23460 (QY488_23460) nadC 5068021..5068869 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QY488_RS23470 (QY488_23470) pilA 5069071..5069520 (-) 450 WP_301461384.1 type 4a pilus biogenesis protein PilA Machinery gene
  QY488_RS23475 (QY488_23475) pilB 5069752..5071452 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QY488_RS23480 (QY488_23480) pilC 5071456..5072676 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  QY488_RS23485 (QY488_23485) pilD 5072680..5073552 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  QY488_RS23490 (QY488_23490) coaE 5073549..5074160 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  QY488_RS23495 (QY488_23495) yacG 5074157..5074357 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  QY488_RS23500 (QY488_23500) - 5074394..5074603 (-) 210 WP_003094660.1 hypothetical protein -
  QY488_RS23505 (QY488_23505) - 5074709..5075398 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  QY488_RS23510 (QY488_23510) - 5075395..5075865 (-) 471 WP_003103866.1 hypothetical protein -
  QY488_RS23515 (QY488_23515) - 5075862..5076287 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  QY488_RS23520 (QY488_23520) - 5076420..5077049 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  QY488_RS23525 (QY488_23525) - 5077046..5077495 (+) 450 WP_003094670.1 MOSC domain-containing protein -
  QY488_RS23530 (QY488_23530) - 5077521..5077691 (+) 171 WP_003094672.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 31869.92 Da        Isoelectric Point: 7.7068

>NTDB_id=853717 QY488_RS23485 WP_003112839.1 5072680..5073552(+) (pilD) [Pseudomonas aeruginosa PAO1]
MPLLDYLASHPLAFVLCTILLGLLVGSFLNVVVHRLPKMMERNWKAEAREALGLEPEPKQATYNLVLPNSACPRCGHEIR
PWENIPLVSYLALGGKCSSCKAAIGKRYPLVELATALLSGYVAWHFGFTWQAGAMLLLTWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIANHFGLFASLDDALFGAVFGYLSLWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AILGVIMLRLRNAESGTPIPFGPYLAIAGWIALLWGDQITRTYLQFAGFK

Nucleotide


Download         Length: 873 bp        

>NTDB_id=853717 QY488_RS23485 WP_003112839.1 5072680..5073552(+) (pilD) [Pseudomonas aeruginosa PAO1]
ATGCCCCTCCTCGACTACCTGGCCAGCCATCCGCTGGCCTTTGTCTTGTGCACCATCCTCCTCGGCCTGCTGGTCGGCAG
CTTCCTCAACGTCGTCGTGCATCGCCTGCCGAAGATGATGGAGCGCAACTGGAAGGCCGAGGCCCGCGAAGCGCTCGGGC
TGGAGCCGGAACCCAAGCAGGCGACCTACAACCTGGTGCTGCCGAATTCGGCGTGCCCGCGCTGCGGCCACGAGATCCGC
CCCTGGGAAAACATCCCGCTGGTCAGCTACCTGGCGCTGGGCGGCAAGTGCTCGTCCTGCAAGGCCGCCATCGGCAAACG
CTACCCCCTGGTGGAACTGGCCACCGCGCTGCTTTCCGGCTACGTCGCCTGGCATTTCGGCTTCACCTGGCAGGCGGGCG
CGATGCTCCTGCTGACCTGGGGTCTGCTGGCGATGAGCCTGATCGATGCCGATCACCAACTGCTGCCGGACGTGCTGGTG
CTGCCGCTGCTCTGGCTGGGCCTGATCGCCAACCATTTCGGCCTCTTCGCCAGCCTCGACGATGCGCTGTTCGGCGCGGT
GTTCGGCTACCTGAGCCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAGGGCATGGGCTACGGCGACT
TCAAGCTGCTGGCCATGCTCGGTGCCTGGGGCGGCTGGCAGATCCTGCCGCTGACCATCCTGCTGTCGTCGCTGGTCGGG
GCGATCCTCGGGGTGATCATGCTGCGCCTGCGCAACGCCGAAAGCGGCACGCCGATCCCCTTCGGTCCCTATCTGGCTAT
TGCCGGGTGGATTGCTTTGCTCTGGGGTGATCAAATAACCCGGACCTATCTGCAATTCGCCGGATTCAAATGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P22610

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.357

96.552

0.534
  pilD Vibrio campbellii strain DS40M4

52.65

97.586

0.514
  pilD Acinetobacter nosocomialis M2

50.53

97.586

0.493
  pilD Acinetobacter baumannii D1279779

50.53

97.586

0.493
  pilD Neisseria gonorrhoeae MS11

50.19

90.69

0.455