Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QUE29_RS18535 Genome accession   NZ_AP027258
Coordinates   3737558..3738235 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain JNE151685     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3732558..3743235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE29_RS18500 (JNE151685_35230) gspG 3733038..3733475 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  QUE29_RS18505 (JNE151685_35240) gspH 3733483..3733992 (+) 510 WP_001076044.1 type II secretion system minor pseudopilin GspH -
  QUE29_RS18510 (JNE151685_35250) gspI 3733989..3734366 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QUE29_RS18515 (JNE151685_35260) gspJ 3734359..3734946 (+) 588 WP_001402582.1 type II secretion system minor pseudopilin GspJ -
  QUE29_RS18520 (JNE151685_35270) gspK 3734939..3735922 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QUE29_RS18525 (JNE151685_35280) gspL 3735937..3737100 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  QUE29_RS18530 (JNE151685_35290) gspM 3737097..3737558 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  QUE29_RS18535 (JNE151685_35300) pilD 3737558..3738235 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QUE29_RS18540 (JNE151685_35310) bfr 3738264..3738740 (-) 477 WP_000675504.1 bacterioferritin -
  QUE29_RS18545 (JNE151685_35320) bfd 3738812..3739006 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QUE29_RS18550 (JNE151685_35330) chiA 3739175..3741868 (-) 2694 WP_001402584.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=85344 QUE29_RS18535 WP_000178161.1 3737558..3738235(+) (pilD) [Escherichia coli strain JNE151685]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=85344 QUE29_RS18535 WP_000178161.1 3737558..3738235(+) (pilD) [Escherichia coli strain JNE151685]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAACTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378

Multiple sequence alignment