NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	QWI19_RS12725	Genome accession	NZ_CP129123
Coordinates	2467836..2468453 (-)	Length	205 a.a.
NCBI ID	WP_004398514.1	Uniprot ID	A0AAE2SLR2
Organism	Bacillus subtilis strain 6D1
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2462836..2473453

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QWI19_RS12695	rpsT	2463138..2463404 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
QWI19_RS12700	holA	2463419..2464462 (-)	1044	WP_015251674.1	DNA polymerase III subunit delta	-
QWI19_RS12705	-	2464502..2464651 (-)	150	WP_003229985.1	hypothetical protein	-
QWI19_RS12710	yqzM	2464692..2464826 (+)	135	WP_003229983.1	YqzM family protein	-
QWI19_RS12715	comEC	2464866..2467196 (-)	2331	WP_015251673.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
QWI19_RS12720	comEB	2467200..2467769 (-)	570	WP_003229978.1	ComE operon protein 2	-
QWI19_RS12725	comEA	2467836..2468453 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
QWI19_RS12730	comER	2468537..2469358 (+)	822	WP_004398597.1	late competence protein ComER	-
QWI19_RS12735	yqeM	2469424..2470167 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
QWI19_RS12740	rsfS	2470164..2470520 (-)	357	WP_003229971.1	ribosome silencing factor	-
QWI19_RS12745	yqeK	2470538..2471098 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
QWI19_RS12750	nadD	2471088..2471657 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
QWI19_RS12755	yhbY	2471669..2471959 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
QWI19_RS12760	aroE	2471953..2472795 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 205 a.a. Molecular weight: 21769.45 Da Isoelectric Point: 4.7220

                                >NTDB_id=851218 QWI19_RS12725 WP_004398514.1 2467836..2468453(-) (comEA) [Bacillus subtilis strain 6D1]

MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT

GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV

GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

Download Length: 618 bp

                                >NTDB_id=851218 QWI19_RS12725 WP_004398514.1 2467836..2468453(-) (comEA) [Bacillus subtilis strain 6D1]

ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT

GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA

ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA

GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA

GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG

TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG

GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT

TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39

Detailed information NTG0851218