Detailed information NTG0850628

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LC071_RS08335
• Genome accession: NZ_CP129020
• Coordinates: 1652983..1653999 (-)
• Length: 338 a.a.
• NCBI ID: WP_370314265.1
• Uniprot ID: -
• Organism: Pseudalkalibacillus hwajinpoensis strain KcN21-2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1647983..1658999

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LC071_RS08315 (LC071_08315)	secA	1648355..1650868 (-)	2514	WP_226615300.1	preprotein translocase subunit SecA	-
LC071_RS08320 (LC071_08320)	hpf	1651088..1651651 (-)	564	WP_194218748.1	ribosome hibernation-promoting factor, HPF/YfiA family	-
LC071_RS08325 (LC071_08325)	-	1651932..1652132 (-)	201	WP_306109609.1	cold shock domain-containing protein	-
LC071_RS08330 (LC071_08330)	-	1652273..1652995 (-)	723	WP_226615303.1	ComF family protein	-
LC071_RS08335 (LC071_08335)	comFA	1652983..1653999 (-)	1017	WP_370314265.1	DEAD/DEAH box helicase	Machinery gene
LC071_RS08340 (LC071_08340)	-	1654498..1655346 (-)	849	WP_226615304.1	DegV family protein	-
LC071_RS08345 (LC071_08345)	-	1655657..1656583 (+)	927	WP_226615305.1	peptidoglycan-binding protein	-
LC071_RS08350 (LC071_08350)	-	1656693..1657398 (-)	706	Protein_1645	response regulator	-
LC071_RS08355 (LC071_08355)	-	1657402..1658553 (-)	1152	WP_226615306.1	sensor histidine kinase	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 38214.27 Da
• Isoelectric Point: 9.9476

>NTDB_id=850628 LC071_RS08335 WP_370314265.1 1652983..1653999(-) (comFA) [Pseudalkalibacillus hwajinpoensis strain KcN21-2]
MSDGQKEASNAVVRAIQHNLILLVWAVCGSGKTEVLFNGLAHALTNQKSVCIATPRTDVVLELAPRLQKVFPSLVISPLY
AGHSDPHSQFVVSTTHQMIRFKNYFDVMIIDEVDAFPYSVDSSLQYNVDKARKQGGSLIYLTATPSPLILKKENLQIVRI
PSRYHGFPLPIPTCHWSGNWRKKINKGHLPNILIQWLKKQVSERKPVLLFLPSIKIINQTKDVFEKNHFRCESVHSEDEN
RAEKVLRFRNKEIPILLSSTILERGITIENISVAVIGAEDEVFTESALVQIAGRAGRSKTFPDGDVRFFHYGKTNAMLEA
IKHIKKMNVEAREKGWLN

Nucleotide

• Length: 1017 bp

>NTDB_id=850628 LC071_RS08335 WP_370314265.1 1652983..1653999(-) (comFA) [Pseudalkalibacillus hwajinpoensis strain KcN21-2]
TTGTCAGATGGTCAAAAGGAAGCTTCTAATGCTGTTGTCAGGGCTATTCAACATAATCTTATTCTCCTTGTATGGGCTGT
TTGTGGTAGTGGTAAAACAGAAGTGTTATTTAATGGATTAGCTCATGCCCTCACCAATCAAAAGTCTGTATGTATTGCTA
CACCACGTACAGATGTTGTACTAGAATTAGCTCCACGCCTACAAAAGGTCTTCCCTAGTCTTGTGATTTCTCCCCTATAT
GCAGGTCATTCCGATCCTCATTCTCAATTTGTTGTTTCTACGACGCATCAAATGATTCGTTTTAAGAATTACTTTGATGT
CATGATCATTGATGAAGTAGATGCGTTTCCATATAGTGTGGATTCTTCTCTTCAATACAACGTTGATAAAGCGAGAAAGC
AAGGCGGTTCTCTCATTTATTTAACCGCAACACCTTCACCGTTGATTTTAAAAAAAGAAAATCTACAAATCGTACGGATA
CCCTCAAGGTACCATGGTTTCCCACTTCCTATCCCAACCTGTCATTGGAGTGGCAATTGGAGAAAGAAAATTAATAAAGG
TCATCTTCCTAATATATTAATTCAATGGCTAAAGAAACAAGTATCTGAGCGAAAGCCCGTCCTCTTATTTCTTCCATCTA
TAAAAATCATTAATCAAACAAAAGATGTATTTGAAAAAAATCACTTCCGATGTGAATCTGTTCATTCTGAGGATGAAAAT
CGAGCCGAAAAAGTTCTCCGTTTTCGAAACAAAGAAATCCCCATTCTTCTTTCATCGACTATTCTCGAGAGAGGCATTAC
GATTGAAAATATATCAGTAGCTGTGATTGGAGCAGAGGATGAGGTATTTACAGAGAGTGCCCTGGTGCAAATTGCCGGAA
GAGCTGGAAGAAGTAAGACATTCCCAGATGGAGATGTTCGCTTTTTTCATTATGGTAAAACAAATGCCATGTTAGAGGCT
ATAAAGCATATTAAGAAAATGAATGTTGAAGCGAGGGAGAAAGGTTGGCTCAATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (3-146)

• Helicase conserved C-terminal domain (Helicase_C) (190-298)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.395	98.817	0.518
comFA/cflA	Streptococcus mitis SK321	40.419	98.817	0.399
comFA/cflA	Streptococcus mitis NCTC 12261	39.222	98.817	0.388
comFA	Lactococcus lactis subsp. cremoris KW2	38.166	100	0.382
comFA/cflA	Streptococcus pneumoniae Rx1	38.623	98.817	0.382
comFA/cflA	Streptococcus pneumoniae D39	38.623	98.817	0.382
comFA/cflA	Streptococcus pneumoniae R6	38.623	98.817	0.382
comFA	Latilactobacillus sakei subsp. sakei 23K	37.758	100	0.379
comFA/cflA	Streptococcus pneumoniae TIGR4	38.323	98.817	0.379